The external horizontal cells (EHCs) in the goldfish retina are known to respond with hyperpolarization to monochromatic flashes of all wavelengths. From their spectral responses they are referred to as "monophasic" horizontal cells. However, when the retina is exposed to an intense red background, EHCs showed a depolarizing response to flashes of intermediate and short wavelength of moderate intensity. The depolarizing component is presumably driven by the input from green-sensitive cones.