Abstract
The Fas antigen (Fas) belongs to the tumor necrosis factor (TNF)/nerve growth factor receptor family, and it mediates apoptosis. Using a soluble form of mouse Fas, prepared by fusion with human immunoglobulin Fc, Fas ligand was detected on the cell surface of a cytotoxic T cell hybridoma, PC60-d10S. A cell population that highly expresses Fas ligand was sorted using a fluorescence-activated cell sorter, and its cDNA was isolated from the sorted cells by expression cloning. The amino acid sequence indicated that Fas ligand is a type II transmembrane protein that belongs to the TNF family. The recombinant Fas ligand expressed in COS cells induced apoptosis in Fas-expressing target cells. Northern hybridization revealed that Fas ligand is expressed in activated splenocytes and thymocytes, consistent with its involvement in T cell-mediated cytotoxicity and in several nonlymphoid tissues, such as testis.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antigens, Surface / biosynthesis*
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Antigens, Surface / genetics*
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Apoptosis*
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Base Sequence
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Cell Line
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Cloning, Molecular
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DNA, Complementary / metabolism
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Flow Cytometry
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Gene Expression
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Humans
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Hybridomas
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Immunoglobulin Fc Fragments / biosynthesis
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Mice
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Molecular Sequence Data
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Multigene Family
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RNA, Messenger / biosynthesis
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Rats
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Receptors, Cell Surface / biosynthesis
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Recombinant Fusion Proteins / biosynthesis
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Sequence Homology, Amino Acid
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T-Lymphocytes, Cytotoxic
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Transfection
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Tumor Necrosis Factor-alpha / genetics*
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fas Receptor
Substances
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Antigens, Surface
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DNA, Complementary
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Immunoglobulin Fc Fragments
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RNA, Messenger
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Receptors, Cell Surface
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Recombinant Fusion Proteins
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Tumor Necrosis Factor-alpha
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fas Receptor
Associated data
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GENBANK/L20821
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GENBANK/L20822
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GENBANK/L20823
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GENBANK/L20888
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GENBANK/L20889
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GENBANK/L33709
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GENBANK/S60904
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GENBANK/S60905
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GENBANK/S60924
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GENBANK/U03470