Abstract
The primary structure of a putative subunit of the mouse glutamate receptor channel, designated as the delta 2 subunit, has been deduced by cloning and sequencing the cDNA. The delta 2 subunit has four putative transmembrane segments characteristic for neurotransmitter-gated ion channels, and shares 56% amino acid sequence identity with the delta 1 subunit of the mouse glutamate receptor channel and 14-24% identity with the subunits of the AMPA-, kainate- or NMDA-selective glutamate receptor channel. RNA blot and in situ hybridization analyses show that the delta 2 subunit mRNA is localized in cerebellar Purkinje cells. Furthermore, immunoblot and immunohistochemical analyses suggest that the delta 2 subunit protein is actually expressed in vivo in Purkinje neurons. The selective localization of the delta 2 subunit in Purkinje cells may imply a role of the delta 2 subunit in Purkinje cell-specific function such as the cerebellar LTD.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Blotting, Northern
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Cell Membrane / metabolism
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Cerebellum / metabolism*
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Cloning, Molecular
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DNA, Complementary / isolation & purification
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Electrophoresis, Polyacrylamide Gel
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Gene Expression
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Immunoblotting
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In Situ Hybridization
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Ion Channels / biosynthesis*
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Ion Channels / isolation & purification
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Macromolecular Substances
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Mice
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Mice, Inbred C57BL
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Molecular Sequence Data
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Polymerase Chain Reaction
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Prosencephalon / metabolism*
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Purkinje Cells / metabolism*
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RNA, Messenger / analysis
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RNA, Messenger / metabolism
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Receptors, Glutamate / biosynthesis*
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Receptors, Glutamate / isolation & purification
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Sequence Homology, Amino Acid
Substances
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DNA, Complementary
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Ion Channels
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Macromolecular Substances
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RNA, Messenger
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Receptors, Glutamate
Associated data
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GENBANK/D13266
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GENBANK/L14791
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GENBANK/L14792
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GENBANK/L14793
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GENBANK/L14794
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GENBANK/L14795
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GENBANK/L14796
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GENBANK/L14797
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GENBANK/L14798
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GENBANK/U03866