Abstract
We report that kainic acid-induced seizures lead to marked increases in mRNAs encoding basic and acidic fibroblast growth factors (bFGF and aFGF, respectively) and flg, one of their receptors, in the rat hippocampus. Anticonvulsant pretreatment inhibits the up-regulation of these mRNAs. The observed increase in flg mRNA levels involves the pyramidal cells of all hippocampal subfields and the granular cells of the dentate gyrus. The increased expression of aFGF and bFGF mRNAs is limited to neuron populations that are resistant to seizure-induced injury, the granular cells of dentate gyrus and pyramidal cells of CA1 region, respectively. The results suggest that the increase in the FGFs and flg may play pivotal roles in neuron survival and in long-term changes occurring in the hippocampus following seizure activity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Diazepam / pharmacology
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Fibroblast Growth Factor 1 / biosynthesis*
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Fibroblast Growth Factor 2 / biosynthesis*
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Hippocampus / cytology
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Hippocampus / drug effects
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Hippocampus / metabolism*
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In Situ Hybridization
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Kainic Acid
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Molecular Sequence Data
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Neurons / drug effects
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Neurons / metabolism
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Oligonucleotide Probes
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Polymerase Chain Reaction
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Pyramidal Cells / drug effects
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Pyramidal Cells / metabolism
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RNA, Messenger / biosynthesis*
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RNA-Directed DNA Polymerase / analysis
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Rats
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Rats, Wistar
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Receptors, Fibroblast Growth Factor / biosynthesis*
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Seizures / chemically induced
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Seizures / metabolism*
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Sulfur Radioisotopes
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Up-Regulation / drug effects
Substances
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Oligonucleotide Probes
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RNA, Messenger
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Receptors, Fibroblast Growth Factor
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Sulfur Radioisotopes
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Fibroblast Growth Factor 2
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Fibroblast Growth Factor 1
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RNA-Directed DNA Polymerase
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Diazepam
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Kainic Acid