Abstract
We have postulated elsewhere (Shaw CA and Lanius RA. Dev Brain Res 70, 153-161 (1992)) that the kinase/phosphatase regulation of AMPA receptors is mediated by specific ions. Using an in vitro cortical slice preparation we have now examined the roles of calcium (Ca2+), chloride (Cl-), potassium (K+), and sodium (Na+) in the regulation of AMPA receptors. Ca2+ led to a concentration-dependent decrease in [3H]-CNQX binding which could be blocked by a general protein kinase inhibitor (H-7) and a protein kinase A inhibiting peptide. Tamoxifen, a relatively specific protein kinase C inhibitor, had no effect. In contrast, Cl- led to concentration-dependent increases in [3H]-CNQX binding which could be blocked by both sodium-ortho-vanadate, a tyrosine residue selective phosphatase inhibitor, and sodium-beta-D-glycerol phosphate, a serine residue selective phosphatase blocker. K+ and Na+ had no effect on [3H]-CNQX binding. These results suggest that Ca2+ and Cl- may be acting as signals which trigger kinase(s) and phosphatase(s) involved in the regulation of AMPA receptors.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
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6-Cyano-7-nitroquinoxaline-2,3-dione
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Animals
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Calcium / metabolism
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Chlorides / metabolism
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Glycerophosphates / pharmacology
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Ibotenic Acid / analogs & derivatives
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Ibotenic Acid / antagonists & inhibitors
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In Vitro Techniques
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Isoquinolines / pharmacology
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Peptides / pharmacology
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Phosphoric Monoester Hydrolases / metabolism*
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Piperazines / pharmacology
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Potassium / metabolism
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Protein Kinase Inhibitors
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Protein Kinases / metabolism*
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Quinoxalines / pharmacology
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Rats
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Rats, Sprague-Dawley
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Receptors, AMPA
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Receptors, Glutamate / metabolism*
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Sodium / metabolism
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Tamoxifen / pharmacology
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Vanadates / pharmacology
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alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
Substances
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Chlorides
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Glycerophosphates
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Isoquinolines
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Peptides
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Piperazines
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Protein Kinase Inhibitors
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Quinoxalines
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Receptors, AMPA
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Receptors, Glutamate
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Tamoxifen
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protein kinase inhibitor peptide
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Ibotenic Acid
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Vanadates
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6-Cyano-7-nitroquinoxaline-2,3-dione
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alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
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1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
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Sodium
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Protein Kinases
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Phosphoric Monoester Hydrolases
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Potassium
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Calcium