Abstract
Furin has been proposed to be the endoprotease responsible for precursor cleavage at Arg-X-Lys/Arg-Arg (RXK/RR) sites within the constitutive secretory pathway. However, there was a possibility that other protease(s) is involved in this cleavage. We here characterized furin in human colon carcinoma LoVo cells, since these cells lacked the endogenous processing activity toward RXK/RR sites and recovered the activity by transfection of furin cDNA. Furin cDNA cloned from LoVo cells had one nucleotide deletion in the region covering the homo B domain which is essential for the endoproteolytic activity. LoVo cells transfected with a furin construct with the mutation showed no activity. Based on these data, we conclude that furin is the endoprotease that is involved in the precursor cleavage at RXK/RR sites within the constitutive secretory pathway.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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CHO Cells
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Cloning, Molecular
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Colonic Neoplasms
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Cricetinae
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Furin
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Humans
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Mice
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Molecular Sequence Data
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Mutagenesis
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Oligodeoxyribonucleotides
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Poly A / isolation & purification
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Poly A / metabolism
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Polymerase Chain Reaction
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Protein Processing, Post-Translational*
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RNA / isolation & purification
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RNA / metabolism
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RNA, Messenger
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Restriction Mapping
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Sequence Homology, Amino Acid
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Sequence Homology, Nucleic Acid
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Substrate Specificity
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Subtilisins / genetics
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Subtilisins / metabolism*
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Transfection
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Tumor Cells, Cultured
Substances
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Oligodeoxyribonucleotides
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RNA, Messenger
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Poly A
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RNA
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Subtilisins
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Furin
Associated data
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GENBANK/D31661
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GENBANK/D37827
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GENBANK/L16877
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GENBANK/L16878
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GENBANK/L18948
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GENBANK/L19108
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GENBANK/L19109
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GENBANK/L19111
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GENBANK/L19112
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GENBANK/S65442