Abstract
The murine D4 dopamine receptor was isolated from a murine genomic DNA library. The receptor's entire coding region was contained within a 6 kb EcoRI genomic fragment, indicating that the murine D4 receptor gene is significantly smaller than the corresponding D2 and D3 receptor genes, the coding regions of which each stretch over 30 kb. The murine D4 receptor gene has three introns and four exons, in common with the rat and human D4 receptor genes. RT-PCR on mRNA from different brain regions shows that the D4 receptor mRNA is expressed in various areas of the brain, with some differences from the rat and human receptor homologues.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Brain / metabolism*
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Cloning, Molecular
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DNA / genetics
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DNA Primers
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Deoxyribonuclease EcoRI
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Exons
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Genomic Library
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Humans
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Introns
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Mice / genetics*
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Molecular Sequence Data
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Organ Specificity
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Polymerase Chain Reaction / methods
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Receptors, Dopamine / biosynthesis*
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Receptors, Dopamine / genetics*
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Receptors, Dopamine D2 / genetics
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Receptors, Dopamine D3
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Receptors, Dopamine D4
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Restriction Mapping
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Sequence Homology, Amino Acid
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Spleen / metabolism
Substances
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DNA Primers
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DRD3 protein, human
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DRD4 protein, human
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Drd3 protein, mouse
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Drd4 protein, mouse
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Receptors, Dopamine
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Receptors, Dopamine D2
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Receptors, Dopamine D3
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Receptors, Dopamine D4
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DNA
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Deoxyribonuclease EcoRI