Abstract
Glycogen synthase kinase 3 (GSK-3) is homologous to the product of the Drosophila gene shaggy (zeste-white 3), which is required for signalling by wingless during Drosophila development. To test whether GSK-3 is also involved in vertebrate pattern formation, its role was investigated during early Xenopus development. It was found that dominant-negative GSK-3 mutants induced dorsal differentiation, whereas wild-type GSK-3 induced ventralization. These results indicate that GSK-3 is required for ventral differentiation, and suggest that dorsal differentiation may involve the suppression of GSK-3 activity by a wingless/wnt-related signal.
MeSH terms
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Animals
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Blastocyst / cytology
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Calcium-Calmodulin-Dependent Protein Kinases / genetics
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Calcium-Calmodulin-Dependent Protein Kinases / physiology*
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Embryo, Nonmammalian / radiation effects
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Embryonic Development*
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Embryonic Induction
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Fibroblast Growth Factor 2 / metabolism
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Glycogen Synthase Kinase 3
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Glycogen Synthase Kinases
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Humans
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Molecular Sequence Data
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Mutation
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RNA, Messenger / metabolism
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Ultraviolet Rays
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Xenopus / embryology
Substances
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RNA, Messenger
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Fibroblast Growth Factor 2
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Glycogen Synthase Kinases
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Calcium-Calmodulin-Dependent Protein Kinases
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Glycogen Synthase Kinase 3
Associated data
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GENBANK/L33801
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GENBANK/L40027