Use of the Pannell-Milstein roller bottle apparatus to produce high concentrations of the CSF-1, the mouse macrophage growth factor

J Immunol Methods. 1995 May 11;182(1):73-9. doi: 10.1016/0022-1759(95)00024-5.

Abstract

A simple roller bottle apparatus for growing B cell hybridomas in dialysis tubing yields high concentrations of monoclonal antibodies (Pannell and Milstein, J. Immunol. Methods (1992) 146, 43-48). Here we describe an adaptation of this apparatus for the simple production of high concentrations of the mouse macrophage specific growth factor, CSF-1. This apparatus appears to have general applicability for the production of other secreted cytokines.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Agar
  • Animals
  • Bone Marrow Cells
  • Clone Cells
  • Culture Techniques / instrumentation
  • Macrophage Colony-Stimulating Factor / analysis
  • Macrophage Colony-Stimulating Factor / biosynthesis*
  • Macrophages / immunology*
  • Mice
  • Mice, Inbred C3H
  • Radioimmunoassay

Substances

  • Macrophage Colony-Stimulating Factor
  • Agar