Abstract
To characterize G-proteins which mediate the signal transduction from ligand stimulated receptor to phospholipase C (PLC), we injected antisense DNAs complementary to Xenopus G(o) alpha or Gi-l alpha to suppress these endogenous G-proteins, together with the mRNAs encoding metabotropic glutamate receptor 1 (mGluR1), 5 (mGluR5) or with M1 type muscarinic receptor into oocytes. Receptor-stimulated chloride current responses were reduced by the suppression of Xenopus G(o) alpha regardless of the types of receptors. However, injection of Gi-1 antisense DNA resulted in the reduction of M1-stimulated responses but not mGluR-stimulated responses. These results suggested that all these receptors could use G(o) alpha, and M1 receptors, but not mGluRs, could also use Gi-1 proteins, to activate PLC in Xenopus oocytes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetylcholine / pharmacology
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Animals
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Base Sequence
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Chloride Channels / metabolism
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Enzyme Activation
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GTP-Binding Proteins / genetics
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GTP-Binding Proteins / metabolism*
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Glutamic Acid / pharmacology
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Inositol 1,4,5-Trisphosphate / metabolism
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Molecular Sequence Data
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Oligonucleotides, Antisense
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Oocytes
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Phosphatidylinositols / metabolism*
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Quinuclidinyl Benzilate / metabolism
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RNA, Messenger / genetics
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Receptors, Metabotropic Glutamate / genetics
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Receptors, Metabotropic Glutamate / metabolism
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Receptors, Muscarinic / genetics
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Receptors, Muscarinic / metabolism
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Signal Transduction
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Type C Phospholipases / metabolism*
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Xenopus
Substances
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Chloride Channels
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Oligonucleotides, Antisense
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Phosphatidylinositols
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RNA, Messenger
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Receptors, Metabotropic Glutamate
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Receptors, Muscarinic
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Glutamic Acid
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Quinuclidinyl Benzilate
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Inositol 1,4,5-Trisphosphate
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Type C Phospholipases
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GTP-Binding Proteins
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Acetylcholine