Abstract
The Id proteins are inhibitors of the basic-helix-loop-helix (bHLH) transcription factors. In the B cell lineage, the Id1 and Id2 genes are expressed in pro-B cells and down-regulated during differentiation. To determine the role of bHLH proteins and the significance of down-regulation of Id genes in B cell development, transgenic mice constitutively expressing the Id1 gene were generated. Their phenotype suggests that B cell development is impaired at an early stage. Primarily, these mice have few B220+ IgM+ mature B or B220+ CD43- pre-B cells in the bone marrow, reduced frequencies of V(D)J and V kappa J kappa recombination of the immunoglobulin loci, and lower expression levels of the immunoglobulin, RAG-1, RAG-2, and lambda 5 genes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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B-Lymphocytes / physiology*
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Cell Differentiation
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DNA-Binding Proteins / biosynthesis*
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Gene Dosage
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Gene Expression Regulation
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Genes, Immunoglobulin / genetics
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Helix-Loop-Helix Motifs*
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Inhibitor of Differentiation Protein 1
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Mice
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Mice, Transgenic
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Recombination, Genetic
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Repressor Proteins*
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TCF Transcription Factors
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Transcription Factor 7-Like 1 Protein
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Transcription Factors / metabolism*
Substances
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DNA-Binding Proteins
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Idb1 protein, mouse
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Inhibitor of Differentiation Protein 1
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Repressor Proteins
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TCF Transcription Factors
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Tcf7l1 protein, mouse
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Transcription Factor 7-Like 1 Protein
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Transcription Factors