Glutamate induces the growth factors NGF, bFGF, the receptor FGF-R1 and c-fos mRNA expression in rat astrocyte culture

Neurosci Lett. 1993 Apr 16;153(1):111-4. doi: 10.1016/0304-3940(93)90089-4.

Abstract

The effect of glutamate on primary cultures of rat cortical astrocytes was studied using Northern blot hybridization. Incubation with glutamate (100 microM, 15 min) induced nerve growth factor (NGF), basic fibroblast growth factor (bFGF), FGF receptor (FGF-R1) and proto-oncogene c-fos gene expression in a time dependent manner. Maximal induction of NGF, bFGF and FGF-R1 mRNA was reached after 4 h of incubation (7.2-fold induction of NGF, 3-fold increase in bFGF and 3.6-fold induction of FGF-R1 mRNA). The induction kinetics of NGF, bFGF and FGF-R1 mRNA are similar. The rapid (1 h) 77-fold induction of the c-fos transcript precedes the induction of the other genes tested.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Astrocytes / metabolism*
  • Blotting, Northern
  • Cells, Cultured
  • Fibroblast Growth Factor 2 / genetics*
  • Gene Expression
  • Glutamates / pharmacology*
  • Glutamic Acid
  • Nerve Growth Factors / genetics*
  • Proto-Oncogene Proteins c-fos / genetics*
  • RNA, Messenger / metabolism*
  • Rats
  • Receptors, Fibroblast Growth Factor / genetics*

Substances

  • Glutamates
  • Nerve Growth Factors
  • Proto-Oncogene Proteins c-fos
  • RNA, Messenger
  • Receptors, Fibroblast Growth Factor
  • Fibroblast Growth Factor 2
  • Glutamic Acid