The in situ size of the dopamine transporter is a tetramer as estimated by radiation inactivation

H E Milner; R Béliveau; S M Jarvis

doi:10.1016/0005-2736(94)90051-5

The in situ size of the dopamine transporter is a tetramer as estimated by radiation inactivation

Biochim Biophys Acta. 1994 Feb 23;1190(1):185-7. doi: 10.1016/0005-2736(94)90051-5.

Authors

H E Milner¹, R Béliveau, S M Jarvis

Affiliation

¹ Research School of Biosciences, University of Kent, Canterbury, UK.

PMID: 8110814
DOI: 10.1016/0005-2736(94)90051-5

Abstract

Radiation inactivation analysis of the mazindol-sensitive binding of the dopamine transporter inhibitor, [3H]GBR-12935 to canine striatal membranes yielded a radiation inactivation target size of 278 +/- 16 kDa. This result, in conjunction with other findings in the literature demonstrating that the molecular mass of the dopamine transporter is approximately 70 kDa, suggests that the native form of the dopamine carrier in the neuronal membranes is a tetrameric assembly of identical 70 kDa subunits.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Carrier Proteins / antagonists & inhibitors
Carrier Proteins / chemistry*
Corpus Striatum / metabolism
Dogs
Dopamine Plasma Membrane Transport Proteins
Membrane Glycoproteins*
Membrane Transport Proteins*
Molecular Weight
Nerve Tissue Proteins*
Piperazines / metabolism
Piperazines / pharmacology*
Protein Binding

Substances

Carrier Proteins
Dopamine Plasma Membrane Transport Proteins
Membrane Glycoproteins
Membrane Transport Proteins
Nerve Tissue Proteins
Piperazines
1-(2 (diphenylmethoxy)ethyl)-4-(3-phenylpropyl)piperazine