Abstract
Targeting of cell ablation agents under the control of tissue-specific promoters promises to be an important tool for studies of development and function in higher organisms. Temperature-sensitive cell ablation agents, recently developed for Drosophila, extend control to temporal as well as spatial aspects of toxin expression. Here we discuss achievements to date, together with a novel form of enhancer trap technology with the potential for driving toxin expression in a large range of cell types.
MeSH terms
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Animals
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Cell Death / genetics*
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Diphtheria Toxin / biosynthesis
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Diphtheria Toxin / genetics
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Diphtheria Toxin / toxicity*
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Drosophila Proteins*
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Drosophila melanogaster / embryology
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Drosophila melanogaster / genetics*
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Embryonic and Fetal Development / drug effects
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Embryonic and Fetal Development / genetics
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Eye Proteins / genetics
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Gene Expression Regulation / drug effects
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Genes, Insect*
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Genes, Suppressor
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Genetic Techniques*
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Membrane Glycoproteins / genetics
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Morphogenesis / drug effects
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Morphogenesis / genetics
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Organ Specificity
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Photoreceptor Cells / metabolism
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Promoter Regions, Genetic*
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Receptor Protein-Tyrosine Kinases*
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Recombinant Fusion Proteins / biosynthesis
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Recombinant Fusion Proteins / toxicity*
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Rhodopsin
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Ricin / biosynthesis
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Ricin / genetics
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Ricin / toxicity*
Substances
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Diphtheria Toxin
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Drosophila Proteins
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Eye Proteins
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Membrane Glycoproteins
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Recombinant Fusion Proteins
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chp protein, Drosophila
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ninaE protein, Drosophila
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Rhodopsin
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Ricin
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Receptor Protein-Tyrosine Kinases
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sev protein, Drosophila