Abstract
A trp E fusion protein containing a C-terminal portion of the rat substance P receptor (SPR) was expressed in bacteria and used to produce an antibody. The antibody specifically reacted with SPR expressed in a mammalian cell line and rat striatum. Light and electron microscope analyses of the rat striatum revealed intense SPR-like immunoreactivity in neuronal somata and dendrites. These immunoreactive neurons constituted approximately 3% of the total population of striatal neurons; they were putative interneurons of large and medium-sized aspiny type.
MeSH terms
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Animals
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Corpus Striatum / cytology
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Corpus Striatum / immunology
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Corpus Striatum / metabolism*
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Dendrites / immunology
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Dendrites / ultrastructure
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Escherichia coli / metabolism
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Genetic Vectors
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Immunohistochemistry
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In Situ Hybridization
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Male
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Microscopy, Electron
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RNA, Messenger / biosynthesis
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Rats
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Rats, Sprague-Dawley
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Receptors, Neurokinin-1
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Receptors, Neurotransmitter / biosynthesis
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Receptors, Neurotransmitter / immunology
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Receptors, Neurotransmitter / metabolism*
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Species Specificity
Substances
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RNA, Messenger
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Receptors, Neurokinin-1
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Receptors, Neurotransmitter