Inter- and intrahemispheric EEG coherence was studied in 4 subjects with agenesis of the corpus callosum (ACC) and in 4 matched controls through different states of the sleep/wakefulness cycle. Interhemispheric coherence was calculated between homologous prefrontal, frontal, central, parietal and occipital electrode pairs whereas intrahemispheric coherence was calculated between all adjacent, unihemispheric electrode pairs. EEG samples were recorded from stage 2, stages 3 + 4 and stage REM sleep and the eyes closed waking state. Interhemispheric coherence measures indicated lower values for ACC subjects than for control subjects for most brain regions; the occipital cortex was least affected. These results further validate the interhemispheric coherence function as a measure of activity in the corpus callosum and suggest that occipital measures may index activity localized in the posterior commissure. Intrahemispheric coherence measures indicated very few differences between the two groups, a result consistent with the suggestion that there is no specialized intrahemispheric compensation in ACC.