Neuronal connectivity of brain areas involved in song learning and song production was studied in the in vitro slice preparation of the zebra finch brain by electrophysiological intracellular recording techniques and by micro-injections of the fluorescent tracer tetramethyl-rhodamine-dextran-amine. While validating some of the known projections to be preserved in the in vitro slice preparation, we were also able to identify a new projection from neurones of the lateral portion of the magnocellular nucleus of the anterior neostriatum towards area X of the lobus parolfactorius.