Abstract
Growth cones and neurites of chicken dorsal root ganglia neurons cultured on laminin, Ng-CAM, or axonin-1 exhibit substratum-dependent morphology and growth patterns which are accompanied by distinctive distributions of axonin-1 and Ng-CAM in the growth cone membrane. On either Ng-CAM or axonin-1 substratum, both Ng-CAM and axonin-1 were depleted from some areas of the apical growth cone membrane. In contrast, on laminin, both axonin-1 and Ng-CAM remained randomly distributed. Removal of axonin-1 from growth cones resulted in a blockage of neurite outgrowth on both Ng-CAM and axonin-1 substrata, indicating that in these neurons axonin-1 cooperates with Ng-CAM in the activation of axon growth. Based on these results possible molecular models for cooperation between axonin-1 and Ng-CAM on the growth cone are discussed.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Blocking
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Axons / physiology*
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Axons / ultrastructure
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Cell Adhesion Molecules, Neuron-Glia / analysis
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Cell Adhesion Molecules, Neuron-Glia / pharmacology
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Cell Adhesion Molecules, Neuron-Glia / physiology*
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Cell Adhesion Molecules, Neuronal / analysis
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Cell Adhesion Molecules, Neuronal / pharmacology
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Cell Adhesion Molecules, Neuronal / physiology*
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Cells, Cultured
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Chick Embryo
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Contactin 2
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Fluorescent Antibody Technique
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Ganglia, Spinal / cytology
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Ganglia, Spinal / embryology
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Immunohistochemistry
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Microscopy, Electron, Scanning
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Microscopy, Immunoelectron
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Neurites / chemistry
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Neurites / drug effects
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Neurites / physiology*
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Neurites / ultrastructure
Substances
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Antibodies, Blocking
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Cell Adhesion Molecules, Neuron-Glia
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Cell Adhesion Molecules, Neuronal
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Contactin 2