Abstract
Leukemia inhibitory factor (LIF) plays an important role in regulating neuropeptide expression in sympathetic and sensory neurons after axonal transection. By 2 h after axotomy, LIF mRNA increased in nonneuronal cells in sympathetic ganglia and peripheral nerve. In addition, within 1 h of explanting sympathetic ganglia or segments of sympathetic nerve trunks, a protein factor(s) that was able to induce LIF mRNA both in sympathetic cultures and in intact ganglia in vivo was released. This factor(s) appeared to be present in sympathetic ganglia and their nerve trunks under normal conditions and to be activated and/or released after axonal injury. Since the factor(s) has a molecular weight(s) greater than 66 kDa, and no other proteins of such high molecular weight have been previously identified with LIF-inducing activity, it appears to be a novel inducer of LIF.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Analysis of Variance
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Animals
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Axons / physiology*
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Cycloheximide / pharmacology
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Galanin / analysis
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Growth Inhibitors / biosynthesis*
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Interleukin-6*
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Leukemia Inhibitory Factor
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Lymphokines / biosynthesis*
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Male
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Nerve Endings / physiology*
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Nerve Tissue Proteins / biosynthesis
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Nerve Tissue Proteins / isolation & purification
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Nerve Tissue Proteins / physiology*
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Organ Culture Techniques
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Protein Synthesis Inhibitors / pharmacology
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RNA, Messenger / biosynthesis
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Rats
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Rats, Sprague-Dawley
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Signal Transduction
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Superior Cervical Ganglion / drug effects
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Superior Cervical Ganglion / physiology*
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Time Factors
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Transcription, Genetic
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Vasoactive Intestinal Peptide / analysis
Substances
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Growth Inhibitors
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Interleukin-6
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Leukemia Inhibitory Factor
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Lymphokines
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Nerve Tissue Proteins
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Protein Synthesis Inhibitors
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RNA, Messenger
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Vasoactive Intestinal Peptide
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Galanin
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Cycloheximide