Abstract
Using a subtractive hybridization technique directed to cloning transcripts with compartmentalized distributions within cerebral cortex neurons, we have isolated rat s-rex mRNAs that are analogues of the human neuroendocrine-specific NSP gene transcripts. Differential splicing produces two main s-rex mRNA that have different regional distributions in the developing and mature rat nervous system. In certain populations of adult brain neurons, most of s-rexs, mRNA and a substantial amount of s-rexb mRNA are localized to the axonal pole of the cell body. The localization of S-Rex/NSP proteins in these neurons suggests that s-rex mRNA compartmentalization targets the encoded proteins to specific regions of the neuron.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alternative Splicing / genetics
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Amino Acid Sequence
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Animals
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Animals, Newborn
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Axons / chemistry
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Blotting, Northern
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Brain / embryology
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Brain / metabolism
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Cell Compartmentation
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Cells, Cultured
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Cerebral Cortex / chemistry*
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Cerebral Cortex / cytology
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Cerebral Cortex / growth & development
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Chickens
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Cloning, Molecular
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DNA, Complementary
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Humans
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In Situ Hybridization
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Mice
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Molecular Sequence Data
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Nerve Tissue Proteins / genetics
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Nerve Tissue Proteins / metabolism*
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Neurons / chemistry*
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RNA, Messenger / analysis
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Rats
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Rats, Wistar
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Sequence Alignment
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Sequence Homology, Amino Acid
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Synaptosomes / chemistry
Substances
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DNA, Complementary
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Nerve Tissue Proteins
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RNA, Messenger
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RTN1 protein, human
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Rtn1 protein, mouse
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Rtn1 protein, rat
Associated data
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GENBANK/U17603
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GENBANK/U17604
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GENBANK/U17605
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GENBANK/U17606