Abstract
Binding sites for different transcription factors have been identified in the regulatory region of the human uPA gene. We investigated the role of NF-kappa B and AP-1 families of transcription factors in the induction of uPA mRNA by TNF-alpha and PMA in the A549 cell line constitutively expressing uPA mRNA and protein. Using the protein synthesis inhibitor cycloheximide and the antioxidant PDTC, that have an opposite effect on NF-kappa B and AP-1 activation, we showed that uPA mRNA induction by TNF-alpha and PMA in the A549 cell line is mainly due to NF-kappa B activation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antioxidants / pharmacology
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Cycloheximide / pharmacology
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DNA / metabolism
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DNA-Binding Proteins / metabolism
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Gene Expression Regulation
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Humans
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I-kappa B Proteins*
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NF-KappaB Inhibitor alpha
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NF-kappa B / metabolism*
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Proline / analogs & derivatives
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Proline / pharmacology
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Protein Binding / drug effects
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Protein Synthesis Inhibitors / pharmacology
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Proto-Oncogene Proteins c-fos / metabolism
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Proto-Oncogene Proteins c-jun / metabolism
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RNA, Messenger
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Recombinant Proteins / pharmacology
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Signal Transduction
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Tetradecanoylphorbol Acetate / pharmacology*
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Thiocarbamates / pharmacology
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Transcription Factor AP-1 / metabolism
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Tumor Cells, Cultured
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Tumor Necrosis Factor-alpha / pharmacology*
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Urokinase-Type Plasminogen Activator / genetics*
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Urokinase-Type Plasminogen Activator / metabolism
Substances
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Antioxidants
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DNA-Binding Proteins
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I-kappa B Proteins
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NF-kappa B
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NFKBIA protein, human
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Protein Synthesis Inhibitors
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Proto-Oncogene Proteins c-fos
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Proto-Oncogene Proteins c-jun
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RNA, Messenger
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Recombinant Proteins
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Thiocarbamates
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Transcription Factor AP-1
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Tumor Necrosis Factor-alpha
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prolinedithiocarbamate
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NF-KappaB Inhibitor alpha
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DNA
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Cycloheximide
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Proline
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Urokinase-Type Plasminogen Activator
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Tetradecanoylphorbol Acetate