Activation of LA-N-2 cell phospholipases by single alanine substitution analogs of amyloid beta peptide (25-35)

I N Singh; K Sato; A Takashima; J N Kanfer

doi:10.1016/s0014-5793(97)00154-3

Activation of LA-N-2 cell phospholipases by single alanine substitution analogs of amyloid beta peptide (25-35)

FEBS Lett. 1997 Mar 17;405(1):65-7. doi: 10.1016/s0014-5793(97)00154-3.

Authors

I N Singh¹, K Sato, A Takashima, J N Kanfer

Affiliation

¹ Department of Biochemistry and Molecular Biology, University of Manitoba, Winnipeg, Canada.

PMID: 9094425
DOI: 10.1016/s0014-5793(97)00154-3

Abstract

A series of single alanine substituted analogs of amyloid beta peptide (25-35) were tested for their ability to activate the phospholipases of cultured LA-N-2 cells. Substitution of alanine for the amino acids 29-34 prevented the activation of phospholipases A2 and D. In addition substitution of alanine at 28 prevented phospholipase D but not phospholipase A2 activation. All the alanine substitutions, except for positions 33 and 35, blunted phospholipase C activations. There were no activations by scrambled amyloid beta peptide.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alanine / metabolism*
Amyloid beta-Peptides / chemistry
Amyloid beta-Peptides / pharmacology*
Enzyme Activation
Humans
Peptide Fragments / chemistry
Peptide Fragments / pharmacology*
Phospholipase D / drug effects*
Phospholipase D / metabolism
Phospholipases A / drug effects*
Phospholipases A / metabolism
Phospholipases A2
Structure-Activity Relationship
Tumor Cells, Cultured
Type C Phospholipases / drug effects*
Type C Phospholipases / metabolism

Substances

Amyloid beta-Peptides
Peptide Fragments
amyloid beta-protein (25-35)
Phospholipases A
Phospholipases A2
Type C Phospholipases
Phospholipase D
Alanine