Abstract
Fluoro-Jade is an anionic fluorochrome capable of selectively staining degenerating neurons in brain slices. The histochemical application of Fluoro-Jade results in a simple, sensitive and reliable method for staining degenerating neurons and their processes. The technique will detect neuronal degeneration resulting from exposure to a variety of neurotoxic insults. Fluoro-Jade can be combined with other fluorescent methodologies including immunofluorescence, fluorescent axonal tract tracing, and fluorescent Nissl counterstaining. Compared to conventional methodologies, Fluoro-Jade is a more sensitive and definitive marker of neuronal degeneration than hematoxylin and eosin (H&E) or Nissl type stains, while being comparably sensitive yet considerably simpler and more reliable than suppressed silver techniques.
MeSH terms
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1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
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Animals
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Antihypertensive Agents
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Axons / chemistry
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Dizocilpine Maleate
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Dopamine Agents
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Eosine Yellowish-(YS)
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Excitatory Amino Acid Agonists
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Excitatory Amino Acid Antagonists
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Eye Enucleation
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Fluorescent Antibody Technique / methods*
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Fluorescent Dyes*
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Hallucinogens
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Hematoxylin
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Ibogaine
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Kainic Acid
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Male
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Metals
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Nerve Degeneration / physiology*
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Nissl Bodies / chemistry
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Nitro Compounds
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Phencyclidine
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Propionates
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Rats
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Rats, Sprague-Dawley
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Sensitivity and Specificity
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Silver Staining
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Stereotaxic Techniques
Substances
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Antihypertensive Agents
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Dopamine Agents
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Excitatory Amino Acid Agonists
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Excitatory Amino Acid Antagonists
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Fluorescent Dyes
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Hallucinogens
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Metals
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Nitro Compounds
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Propionates
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Ibogaine
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Dizocilpine Maleate
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1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
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Phencyclidine
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3-nitropropionic acid
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Kainic Acid
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Eosine Yellowish-(YS)
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Hematoxylin