Abstract
Rapid flow of information in the nervous system involves presynaptic vesicle recycling by clathrin-mediated endocytosis, an event triggered by the alpha-adaptin-containing AP2 complex. We identified a Drosophila alpha-adaptin expressed in the garland cells, imaginal discs, and the CNS. Here we show its role in presynaptic vesicle recycling. In presynaptic terminals, alpha-adaptin defines a network-like membrane structure to which the GTPase dynamin is recruited. alpha-adaptin is necessary for the formation of clathrin-coated pits and participates in the dynamin-dependent release of coated vesicles from the membrane surface. Our results suggest an alpha-adaptin-dependent control of the vesicle cycle that maintains the balance between the amount of vesicle- and surface-associated membranes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Protein Complex 2
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Adaptor Protein Complex alpha Subunits
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Adaptor Proteins, Vesicular Transport
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Animals
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Antibody Specificity
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Drosophila
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Dynamins
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GTP Phosphohydrolases / genetics
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GTP Phosphohydrolases / metabolism
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Gene Expression / physiology
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Genes, Insect / physiology
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In Situ Hybridization
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Larva / chemistry
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Larva / physiology
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Membrane Proteins / genetics*
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Membrane Proteins / immunology
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Membrane Proteins / metabolism
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Microscopy, Electron
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Microtubules / genetics
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Microtubules / metabolism
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Molecular Sequence Data
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Mutation / physiology
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Presynaptic Terminals / chemistry
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Presynaptic Terminals / metabolism*
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Presynaptic Terminals / ultrastructure
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RNA, Messenger / analysis
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Rabbits
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Sequence Homology, Amino Acid
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Synaptic Vesicles / chemistry
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Synaptic Vesicles / metabolism*
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Synaptic Vesicles / ultrastructure
Substances
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Adaptor Protein Complex 2
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Adaptor Protein Complex alpha Subunits
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Adaptor Proteins, Vesicular Transport
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Membrane Proteins
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RNA, Messenger
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GTP Phosphohydrolases
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Dynamins