Processing of Alzheimer's amyloid precursor protein during H2O2-induced apoptosis in human neuronal cells

L Zhang; B Zhao; D T Yew; J W Kusiak; G S Roth

doi:10.1006/bbrc.1997.6698

Processing of Alzheimer's amyloid precursor protein during H2O2-induced apoptosis in human neuronal cells

Biochem Biophys Res Commun. 1997 Jun 27;235(3):845-8. doi: 10.1006/bbrc.1997.6698.

Authors

L Zhang¹, B Zhao, D T Yew, J W Kusiak, G S Roth

Affiliation

¹ Laboratory of Cellular and Molecular Biology, Gerontology Research Center, NIA/NIH, Baltimore, Maryland, USA.

PMID: 9207249
DOI: 10.1006/bbrc.1997.6698

Abstract

The processing of Alzheimer's amyloid precursor protein was studied by Western blotting during H2O2 induced apoptosis in cultures of human neuroblastoma cells. A new 5.5 kDa fragment putatively containing intact A beta was detected and found to be highly associated with apoptosis. The results suggest a possible vicious cycle involving H2O2, A beta and apoptosis which may contribute to the neuronal death mechanism in Alzheimer's Disease.

MeSH terms

Alzheimer Disease
Amyloid beta-Peptides / biosynthesis
Amyloid beta-Protein Precursor / metabolism*
Apoptosis / drug effects
Apoptosis / physiology*
Humans
Hydrogen Peroxide / pharmacology*
Models, Neurological
Neuroblastoma
Neurons / cytology
Neurons / drug effects
Neurons / metabolism*
Peptide Fragments / analysis
Peptide Fragments / metabolism
Polymerase Chain Reaction
Protein Processing, Post-Translational / drug effects*
Tumor Cells, Cultured

Substances

Amyloid beta-Peptides
Amyloid beta-Protein Precursor
Peptide Fragments
Hydrogen Peroxide