Modification of a PCR-based site-directed mutagenesis method

Biotechniques. 1997 Oct;23(4):570-1, 574. doi: 10.2144/97234bm01.

Authors

C L Fisher¹, G K Pei

Affiliation

¹ NCI-FCRDC, Frederick, MD 21702, USA. fisherc@ncifcrf.gov

PMID: 9343663
DOI: 10.2144/97234bm01

No abstract available

MeSH terms

DNA, Complementary
Deoxyribonucleases, Type II Site-Specific / metabolism
Genetic Vectors
Humans
Mutagenesis, Site-Directed*
Polymerase Chain Reaction / methods*
Proto-Oncogene Proteins / genetics*
Proto-Oncogene Proteins c-ets
Saccharomyces cerevisiae / genetics
Transcription Factors / genetics*

Substances

DNA, Complementary
Proto-Oncogene Proteins
Proto-Oncogene Proteins c-ets
Transcription Factors
endodeoxyribonuclease DpnI
Deoxyribonucleases, Type II Site-Specific