Abstract
We describe a strategy for generating an allelic series of mutations at a given locus that requires the production of only one targetted mouse line. The 'allelogenic' mouse line we produced carries a hypomorphic allele of Fgf8, which can be converted to a null allele by mating to cre transgenic animals. The hypomorphic allele can also be reverted to wild-type by mating the allelogenic mice to flp transgenic animals, thereby generating a mouse line suitable for Cre-induced tissue-specific knockout experiments. Analysis of embryos carrying different combinations of these alleles revealed requirements for Fgf8 gene function during gastrulation, as well as cardiac, craniofacial, forebrain, midbrain and cerebellar development.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Actins / genetics
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Alleles
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Animals
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Congenital Abnormalities / embryology
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Congenital Abnormalities / genetics*
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Crosses, Genetic
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DNA Nucleotidyltransferases / metabolism*
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Embryonic and Fetal Development
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Fibroblast Growth Factor 8
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Fibroblast Growth Factors*
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Gene Library
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Growth Substances / biosynthesis
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Growth Substances / genetics*
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Humans
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Integrases / metabolism*
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Mice
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Mice, Transgenic
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Polymerase Chain Reaction
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Promoter Regions, Genetic
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Recombination, Genetic*
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Viral Proteins*
Substances
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Actins
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FGF8 protein, human
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Fgf8 protein, mouse
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Growth Substances
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Viral Proteins
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Fibroblast Growth Factor 8
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Fibroblast Growth Factors
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Cre recombinase
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DNA Nucleotidyltransferases
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FLP recombinase
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Integrases