To understand the role of the ipsilateral motor cortex in the control of unimanual movements, we evaluated changes in cortical motor evoked potentials (MEP) from the left abductor pollici brevis (APB) to transcranial magnetic stimulation (TMS) of the right hemisphere in nine normal subjects during execution of right finger movements of different complexities. The motor tasks were (a) repetitive opposition movement (thumb tapping the 3rd finger); (b) isolated finger movements in a 'usual' sequence (thumb tapping fingers 2, 3, 4 and 5) and (c) in an 'unusual' sequence (thumb tapping fingers 3, 5, 2 and 4). Subjects were trained before the study up to disappearance of EMG synkinetic activity in the left APB. As compared to the rest condition, MEP amplitude was enhanced in all subjects during paradigm (b) and even more during (c), but remained unchanged during paradigm (a). The MEP increase disappeared in four out of the nine subjects undergoing overtraining. No significant modifications in MEP amplitude were found in the left proximal muscle (biceps, five subjects). The H reflex induced by left median nerve stimulation at the elbow (four subjects) and MEPs from the left APB to transcranial electrical stimulation (three subjects) were not significantly affected by any of the motor paradigms, indicating that the motor cortex was the site of change. These results provide evidence of an increased excitability of cortical motor outputs targeting the unmoving hand muscles during contralateral sequential finger movements which disappears with overtraining. We conclude that during motor learning there is an interhemispheric transfer of information, possibly in order to inhibit the opposite hemisphere from interfering when a fine unimanual movement is required.