Abstract
Glutamate transport is a primary mechanism for the synaptic inactivation of glutamate. Excitatory amino acid transporter 4 (EAAT4) is a novel glutamate transporter with properties of a ligand-gated chloride channel that was recently cloned from human brain. Here we report the cloning of rat EAAT4 (rEAAT4) cDNA from rat cerebellum. The nucleotide sequence of rEAAT4 was 88% identical to the human sequence, and the predicted peptide was 89% identical to the human protein. The transport activity encoded by rEAAT4 has high affinity for L-glutamate. In Xenopus laevis oocytes expressing rEAAT4, L-glutamate and other transporter substrates elicited a current predominantly carried by chloride ions. Like human EAAT4, the rEAAT4 mRNA was largely restricted to cerebellar Purkinje cells; the rEAAT4 protein was localized to Purkinje cell somas and dendrites.
Copyright 1998 Elsevier Science B.V.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Transport System X-AG*
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Animals
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Chloride Channels / genetics*
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Chloride Channels / metabolism
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Chlorides / metabolism
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Cloning, Molecular
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DNA, Complementary
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Electric Stimulation
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Electrophysiology
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Excitatory Amino Acid Transporter 4
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Gene Expression / physiology
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Glutamate Plasma Membrane Transport Proteins
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Glutamic Acid / pharmacology
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Ion Channel Gating / drug effects
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Ligands
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Membrane Potentials / drug effects
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Membrane Potentials / physiology
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Molecular Sequence Data
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Oocytes / physiology
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Purkinje Cells / chemistry*
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Purkinje Cells / physiology*
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Rats
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Receptors, Glutamate / genetics*
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Receptors, Glutamate / metabolism
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Sequence Homology, Amino Acid
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Symporters*
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Xenopus laevis
Substances
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Amino Acid Transport System X-AG
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Chloride Channels
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Chlorides
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DNA, Complementary
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Excitatory Amino Acid Transporter 4
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Glutamate Plasma Membrane Transport Proteins
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Ligands
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Receptors, Glutamate
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Slc1a6 protein, rat
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Symporters
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Glutamic Acid