Abstract
We recently identified the required collaborative signaling of TGFalpha and collagen type IV to regulate cell fate choice in the cerebral cortex, measured by the expression of the limbic system associated membrane protein (LAMP) by nonlimbic, sensorimotor progenitors. We show that activation of different members of the erbB receptor family can similarly modulate the specification of cortical area fate. The region of the cerebral wall from which progenitor cells arise does not influence the response to the neuregulin-1 or TGFalpha, but a subpopulation of progenitors is not competent to express LAMP in response to neuregulin-1. The heterogeneity in the responsiveness by progenitors to the two growth factors is reflected in the expression of different repertoires of erbB receptors. Using clonal analysis, we demonstrate that there may be a lineage-dependent mechanism regulating the ability of neuronal progenitors to respond to specific inductive cues that control cell fate.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antineoplastic Agents / pharmacology
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Cell Adhesion Molecules, Neuronal / genetics
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Cell Cycle / drug effects
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Cell Cycle / physiology
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Cell Differentiation / drug effects
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Cell Differentiation / physiology
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Cell Lineage / physiology
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Cerebral Cortex / chemistry
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Cerebral Cortex / cytology*
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Collagen / pharmacology
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ErbB Receptors / genetics
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Female
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GPI-Linked Proteins
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Gene Expression Regulation, Developmental
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Glycoproteins / pharmacology
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Ligands
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Limbic System / cytology
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Neuregulins
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Neurons, Afferent / chemistry*
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Neurons, Afferent / cytology
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Neurons, Afferent / drug effects
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Oncogene Proteins v-erbB / analysis
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Oncogene Proteins v-erbB / genetics*
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Pregnancy
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Rats
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Rats, Sprague-Dawley
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Signal Transduction / drug effects
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Signal Transduction / physiology*
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Stem Cells / chemistry*
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Stem Cells / cytology
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Stem Cells / drug effects
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Transforming Growth Factor alpha / pharmacology
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Visual Pathways / chemistry
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Visual Pathways / cytology
Substances
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Antineoplastic Agents
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Cell Adhesion Molecules, Neuronal
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GPI-Linked Proteins
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Glycoproteins
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Ligands
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Neuregulins
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Oncogene Proteins v-erbB
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Transforming Growth Factor alpha
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limbic system-associated membrane protein
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Collagen
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ErbB Receptors