[HTML][HTML] Luminescent proteins for high-speed single-cell and whole-body imaging
…, M Hashida, Y Yoshida, T Matsuda, Y Arai… - Nature …, 2012 - nature.com
The use of fluorescent proteins has revolutionized our understanding of biological processes.
However, the requirement for external illumination precludes their universal application to …
However, the requirement for external illumination precludes their universal application to …
Improved Orange and Red Ca2+ Indicators and Photophysical Considerations for Optogenetic Applications
…, M Drobizhev, YF Chang, S Araki, Y Arai… - ACS chemical …, 2013 - ACS Publications
We have used protein engineering to expand the palette of genetically encoded calcium ion
(Ca 2+ ) indicators to include orange and improved red fluorescent variants, and validated …
(Ca 2+ ) indicators to include orange and improved red fluorescent variants, and validated …
[HTML][HTML] Five colour variants of bright luminescent protein for real-time multicolour bioimaging
…, T Kimura, H Shinoda, G Bai, MJ Daniels, Y Arai… - Nature …, 2016 - nature.com
Luminescence imaging has gained attention as a promising bio-imaging modality in situations
where fluorescence imaging cannot be applied. However, wider application to multicolour …
where fluorescence imaging cannot be applied. However, wider application to multicolour …
Self-organization of the phosphatidylinositol lipids signaling system for random cell migration
Y Arai, T Shibata, S Matsuoka… - Proceedings of the …, 2010 - National Acad Sciences
Phosphatidylinositol (PtdIns) lipids have been identified as key signaling mediators for random
cell migration as well as chemoattractant-induced directional migration. However, how …
cell migration as well as chemoattractant-induced directional migration. However, how …
[HTML][HTML] SuperNova, a monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation
…, N Sakai, D Fu, M Noda, S Uchiyama, I Kotera, Y Arai… - Scientific reports, 2013 - nature.com
Chromophore-assisted light inactivation (CALI) is a powerful technique for acute perturbation
of biomolecules in a spatio-temporally defined manner in living specimen with reactive …
of biomolecules in a spatio-temporally defined manner in living specimen with reactive …
[HTML][HTML] Genetically encoded ratiometric fluorescent thermometer with wide range and rapid response
Temperature is a fundamental physical parameter that plays an important role in biological
reactions and events. Although thermometers developed previously have been used to …
reactions and events. Although thermometers developed previously have been used to …
A fast-and positively photoswitchable fluorescent protein for ultralow-laser-power RESOLFT nanoscopy
Fluorescence nanoscopy has revolutionized our ability to visualize biological structures not
resolvable by conventional microscopy. However, photodamage induced by intense light …
resolvable by conventional microscopy. However, photodamage induced by intense light …
[HTML][HTML] Genetically encoded bioluminescent voltage indicator for multi-purpose use in wide range of bioimaging
S Inagaki, H Tsutsui, K Suzuki, M Agetsuma, Y Arai… - Scientific reports, 2017 - nature.com
We report development of the first genetically encoded bioluminescent indicator for membrane
voltage called LOTUS-V. Since it is bioluminescent, imaging LOTUS-V does not require …
voltage called LOTUS-V. Since it is bioluminescent, imaging LOTUS-V does not require …
[PDF][PDF] Importin alpha subtypes determine differential transcription factor localization in embryonic stem cells maintenance
N Yasuhara, R Yamagishi, Y Arai, R Mehmood… - Developmental Cell, 2013 - cell.com
We recently demonstrated that the expression of the importin α subtype is switched from α2
to α1 during neural differentiation in mouse embryonic stem cells (ESCs) and that this …
to α1 during neural differentiation in mouse embryonic stem cells (ESCs) and that this …
Rapid Severing and Motility of Chloroplast-Actin Filaments Are Required for the Chloroplast Avoidance Response in Arabidopsis
SG Kong, Y Arai, N Suetsugu, T Yanagida… - The Plant …, 2013 - academic.oup.com
Phototropins (phot1 and phot2 in Arabidopsis thaliana) relay blue light intensity information
to the chloroplasts, which move toward weak light (the accumulation response) and away …
to the chloroplasts, which move toward weak light (the accumulation response) and away …