Abstract
Homer1 mutant mice exhibit behavioral and neurochemical abnormalities that are consistent with an animal model of schizophrenia. Because the Homer1 gene encodes both immediate early gene (IEG) and constitutively expressed (CC) gene products, we used the local infusion of adeno-associated viral vectors carrying different Homer1 transcriptional variants into the prefrontal cortex (PFC) to distinguish between the roles for IEG and CC Homer1 isoforms in the “schizophrenia-like” phenotype of Homer1 mutant mice. PFC overexpression of the IEG Homer1 isoform Homer1a reversed the genotypic differences in behavioral adaptation to repeated stress, whereas overexpression of the constitutively expressed Homer1 isoform Homer1c reversed the genotypic differences in sensorimotor and cognitive processing, as well as cocaine behavioral sensitivity. Homer1a overexpression did not influence PFC basal glutamate content but blunted the glutamate response to cocaine in wild-type mice. In contrast, Homer1c overexpression reversed the genotypic difference in PFC basal glutamate content and enhanced cocaine-induced elevations in glutamate. These data demonstrate active and distinct roles for Homer1a and Homer1c isoforms in the PFC in the mediation of behavior, in the maintenance of basal extracellular glutamate, and in the regulation of PFC glutamate release relevant to schizophrenia and stimulant abuse comorbidity.
Introduction
Abnormalities in frontal cortical glutamate are implicated in the pathophysiology of schizophrenia (Tsai and Coyle, 2002; Krystal et al., 2003). Linkage analyses identified a single nucleotide polymorphism in the intronic sequence of Homer1 (IVS4 + 18A > G) associated with this disorder (Norton et al., 2003). Constitutively expressed forms of Homer1 (Homer1b, Homer1c, Homer1d) contain an N-terminal Ena/VASP1 Homology (EVH1) domain and a C-terminal coiled-coil (CC) domain and function to: (1) increase group 1 metabotropic glutamate receptor (mGluR) coupling to inositol triphosphate and ryanodine receptors (Ango et al., 2001; Fagni et al., 2002; Feng et al., 2002) and to phosphatidyl inositol 3 kinase (Rong et al., 2003); (2) regulate cation influx through transient receptor potential channels (Yuan et al., 2003) and N-type calcium and M-type potassium channels (Kammermeier et al., 2000); and (3) cross-link other proteins associated with the NMDA subtype of ionotropic glutamate receptors (Naisbitt et al., 1999; Tu et al., 1999) to promote efficient excitatory signaling complexes (Xiao et al., 2000; Fagni et al., 2002). In contrast to constitutively expressed Homer proteins (CC-Homers), ania-3 and Homer1a are Homer1 transcriptional variants that exhibit an immediate early gene (IEG) profile of expression after synaptic activation (Brakeman et al., 1997; Kato et al., 1998; Bottai et al., 2002). IEG Homer1 isoforms possess the EVH1 domain but lack the CC motif required for Homer–Homer multimerization (Bottai et al., 2002). IEG Homers thereby function as natural dominant negatives that disrupt CC-Homer interactions with EVH1-bound proteins (Xiao et al., 2000; Fagni et al., 2002), resulting in a modification of synaptic architecture (Sala et al., 2001; 2003), redistribution of CC-Homer expression (Inoue et al., 2004), and an alteration in excitatory synaptic transmission (Abe et al., 2003; Hennou et al., 2003; Minami et al., 2003).
Homer1 knock-out (KO) mice exhibit a “schizophrenia-like” behavioral phenotype characterized by heightened anxiety and “behavioral despair,” impaired sensorimotor, cognitive, and motivational processing as well as increased behavioral sensitivity to both psychomotor stimulant and dissociative anesthetic drugs (Szumlinski et al., 2004, 2005a). Moreover, acute administration of the typical antipsychotic haloperidol completely reverses the genotypic differences in sensorimotor function (Szumlinski et al., 2005a). Homer1 KO mice exhibit an ∼50% increase in basal extracellular levels of prefrontal cortex (PFC) glutamate and a reduced capacity of cocaine to elevate PFC glutamate levels, suggesting hypo-frontality (Szumlinski et al., 2005a). Because Homer1 gene deletion eliminates both IEG and CC-Homer products, we infused adeno-associated viral vectors (AAVs) carrying either Homer1a or Homer1c into the PFC to address the relative role for these different Homer1 isoforms in the “schizophrenia-like” behavioral and neurochemical phenotype of Homer1 KO mice. Because AAV-mediated upregulation of Homer1c, but not Homer1a, reverses the expression of behavioral and glutamate sensitization in a cocaine-sensitized animal model of psychosis (Szumlinski et al., 2005c), it was hypothesized that the PFC upregulation of Homer1c would reverse the differences in cognitive, sensorimotor, and emotional processing as well as cocaine sensitivity observed between Homer1 wild-type (WT) and KO mice. The present results confirm active, but distinct, roles for PFC Homer1a and Homer1c expression in contributing to the behavioral phenotype of Homer1 KO mice and implicate the modulation of PFC basal and evoked glutamate transmission by Homer1c in normal cognitive and sensorimotor function. Moreover, these data implicate Homer-associated intracellular signaling mechanisms as potential targets for the development of novel pharmacotherapeutics for the treatment of psychotic disorders.
Materials and Methods
Animals. Subjects were WT and Homer1 KO mice [cytomegalovirus (CMV)-Cre (BALB/cJ) × Flpe (C57BL/6 × 129sv) × C57BL/6] (Szumlinski et al., 2004, 2005a). Mice were housed individually in an Association for Assessment and Accreditation of Laboratory Animal Care-approved animal facility in standard mouse cages (lights on at 8:00 A.M.; 25°C; food and water available ad libitum). All testing was conducted during the light cycle. All experiments were approved by the Institutional Animal Care and Use Committee of the Medical University of South Carolina and conducted in accordance with the National Institutes of Health Principles of Laboratory Animal Care. Because insufficient numbers of one gender were available at the time of study, both male and female mice were used. As per our previous results, no gender differences were observed for any of the variables examined (no main effects of, or interactions with, the gender factor; p > 0.05).
Construction of AAV. Because Homer1c is the predominant CC-Homer isoform in rodents, the rat Homer1a and Homer1c coding sequences were amplified using whole-brain cDNA, and the PCR was product expressed as an N-terminal fusion protein with the hemagglutinin (HA)-tag in a rAAV backbone containing the 1.1 kb CMV enhancer/chicken-actin (CBA) promoter, 800 bp human interferon scaffold attachment region inserted 5_ of the promoter, the woodchuck posttranscriptional regulatory element (WPRE), and the bovine growth hormone polyA flanked by inverted terminal repeats (AAV-Homer1a, AAV-Homer1c). The same AAV-CBA-WPRE-bGH backbone encoding the enhanced green fluorescent protein (EGFP) was used as control (AAV-GFP). AAV pseudotyped vectors (virions containing a 1:1 ratio of AAV1 and AAV2 capsid proteins with AAV2 intertrigeminal regions were generated as described previously (Hauck et al., 2003). Briefly, human embryonic kidney 293 cells were transfected with the AAV cis-plasmid, the AAV1 (pH21) and AAV2 (pRV1) helper plasmids (pF6), and the adenovirus helper plasmid by standard calcium phosphate transfection methods. Forty-eight hours after transfection, cells were harvested and the vector purified using heparin affinity columns as described previously (During et al., 2003). Genomic titers were determined using the Prism 7700 sequence detector system (Applied Biosystems, Foster City, CA) with primers designed to WPRE as described previously (Clark et al., 1999).
Surgical procedures and AAV infusion. Under pentobarbital anesthesia (50 mg/kg, i.p.), naive mice (5–7 weeks of age) were implanted bilaterally with a 20-gauge stainless steel guide cannula (20 mm long) aimed 3 mm above the prefrontal cortex (anteroposterior, +1.34 mm; mediolateral, ±0.6 mm; dorsoventral, –2.0 mm; relative to bregma), and the guide cannulas were fixed to the skull using dental resin as described previously (Szumlinski et al., 2004, 2005a,b). After atleast 7 d of recovery, mice were infused bilaterally at a rate of 0.05 μl/min for 5 min (total volume, 0.25 μl/side) with either an AAV carrying the Homer1a or the Homer1c transcriptional variant of the Homer1 gene or an AAV carrying EGFP as a control (Szumlinski et al., 2004, 2005b). Behavioral testing commenced at 3 weeks after infusion. AAV transfection and localization was verified by immunostaining for the HA tag as described previously (Szumlinski et al., 2004, 2005b,c; Klugmann et al., 2005).
Behavioral testing battery. AAV-infused mice were subjected to a battery of behavioral tests to delineate the relative contribution of Homer1a and Homer1c to the abnormal behavioral phenotype of Homer1 KO mice. Unless otherwise indicated, all tests were conducted similarly as described in our original characterization of the Homer1 KO phenotype (Szumlinski et al., 2005a). In all, five replicates of 12–18 animals were examined, the order of behavioral testing was varied slightly across replicates, and 4–5 d separated each behavioral test. Prepulse inhibition (PPI) was conducted toward the end of the third week after AAV infusion in a manner identical to that described in detail previously (Szumlinski et al., 2005a). Animals were then assessed for behavioral despair in a Porsolt swim test (one 15 min exposure, followed 24 h later by a 5 min test) or for working memory in a water version of the radial arm maze (14 d of training with four 2 min trials per day) (Szumlinski et al., 2005a). Animals were next assessed for locomotor activity in response to being placed into a novel activity monitor (30 min session) and then for the habituation of locomotor reactivity after four subsequent daily exposures. Consistent with a relatively high prevalence of comorbidity between schizophrenia and stimulant abuse disorders (Batel, 2000; Gerber and Tonegawa, 2004), Homer1 KO mice exhibit enhanced behavioral sensitivity to the rewarding and psychomotor-activating effects of cocaine (Szumlinski et al., 2004). Thus, the last behavioral experiment assessed cocaine-induced locomotion and used a repeated measures design in which all mice received one of four cocaine doses (0, 3, 10, and 30 mg/kg, i.p.; volume, 0.01 ml/g) in a counterbalanced manner on tests separated by 2–3 d. No habituation period proceeded the cocaine injection sessions, and mice were monitored for 60 min after injection.
In vivo microdialysis and HPLC procedures. No net-flux and conventional microdialysis were conducted as described previously (Szumlinski et al., 2004, 2005a,c). A within-subjects design was used in which mice were first tested using the no-flux technique and then conventional microdialysis was conducted 4–5 d later using the contralateral side of the head. For the no net-flux study, increasing concentrations of glutamate (0, 2.5, 5, and 10 μm) were infused through the probe for 1 h for each concentration. For the cocaine study, baseline samples were collected for 60 min before an intraperitoneal injection of 10 mg/kg cocaine (volume, 0.01 ml/g) and then for 120 min thereafter. Glutamate in the dialysis sample was measured using an HPLC system with fluorescent detection in a manner identical to that described previously (Szumlinski et al., 2004, 2005a,b,c).
Statistical analyses. It was hypothesized that AAV-Homer1c infusion would selectively reverse the differences in behavior and PFC glutamate observed between Homer1 WT and KO mice, and thus all univariate data were analyzed using planned orthogonal comparisons between WT and KO mice within each AAV treatment (Glass and Hopkins, 1984). For data involving repeated measures (cocaine-induced locomotion and changes in extracellular glutamate), the data were analyzed using an analysis of variance. If significant interactions were found, the data were decomposed for main effects of genotype. Because multiple comparisons using one group were not conducted, α = 0.05 for all comparisons. The results of these analyses are presented in Table 1.
Results
Verification of PFC transfection by AAV
To delineate the roles for Homer1a and Homer1c in the PFC phenotype of Homer1 KO mice, groups of WT and KO mice were bilaterally infused intra-PFC with AAVs carrying Homer1a, Homer1c, or GFP control cDNA. Both Homer1a and Homer1c were HA-tagged, and AAV transfection was verified by immunostaining after completion of experimentation (at 8–10 weeks after infusion) (Fig. 1a,b). In both cases, cellular transfection was restricted to an area of ∼1–1.5 mm around the injection/probe site, which was located in the dorsal PFC, and Homer protein expression was localized to both processes and soma of pyramidal neurons (Fig. 1a',b').
AAV-GFP infusion does not alter the behavioral or neurochemical abnormalities produced by Homer1 deletion
Compared with WT mice, Homer1 KO mice exhibit abnormalities in working memory, sensorimotor gating, and emotional reactivity that are associated with increased basal extracellular levels of glutamate in the PFC as well as a reduction in stimulated glutamate release in this brain region (Szumlinski et al., 2005a). Consistent with the results of our previous report, similar differences in behavior and PFC extracellular levels of glutamate were observed between WT and KO mice infused intra-PFC with AAV-GFP control (Table 1). KO control mice exhibited the following: (1) impairments in the acquisition of a radial arm maze and deficits in prepulse inhibition of acoustic startle (Fig. 2); (2) increased behavioral despair in a Porsolt swim test as well as heightened behavioral reactivity to a novel environment and impaired behavioral habituation to that environment (Fig. 3); (3) increased cocaine-induced locomotion (Fig. 4a); (4) elevated basal extracellular levels of PFC glutamate (Fig. 5; Tables 1, 2); and (5) a blunted capacity of cocaine to elevate PFC extracellular glutamate levels above baseline (Fig. 6a). Collectively, these data indicate that an intra-PFC infusion of AAV-GFP did not influence the abnormal behavioral and PFC glutamate phenotype of Homer1 KO mice.
AAV-Homer1c reverses genotype differences in working memory and sensorimotor function
The effects of Homer1a and Homer1c overexpression in the PFC were assessed on performance in a water version of the radial arm maze by examining the change in latency to correctly navigate the maze, the change in the number of working memory errors committed during maze navigation, and the sensitization of “chaining” behavior (repeated entries into adjacent arms) across maze task acquisition (Fig. 2a–c). Whereas a genotypic difference was observed for all of these variables in Homer1a-infused mice, no difference was observed between WT and KO mice infused with Homer1c (Table 1). Thus, PFC overexpression of Homer1c reversed the deficits in working memory observed in Homer1 KO mice, indicating an active role for PFC Homer1c in this form of cognitive processing.
The effects of overexpression of Homer1 isoforms were also assessed on the capacity of a 90 dB prepulse to inhibit the startle response to a 110 dB acoustic stimulus. As illustrated in Figure 2d, a genotypic difference was observed in Homer1a-infused mice, but no difference in prepulse inhibition was observed between WT and KO mice infused with Homer1c (Table 1). Thus, PFC Homer1c actively regulates sensorimotor processing.
AAV-Homer1a reverses genotypic differences in emotional reactivity
To assess for the effects of Homer1a and Homer1c upregulation after behavioral despair, we used a Porsolt swim test. Mice were subjected to a 15 min forced swim exposure and then examined for differences in floating behavior 24 h later. There was a marked genotypic difference between Homer1c-infused mice regarding both the latency to first exhibit floating (Fig. 3a) and the total amount of floating exhibited by the mice (Fig. 3b). In contrast to the data for the radial arm maze and prepulse inhibition above, no genotypic difference was apparent for either variable for mice infused with Homer1a (Table 1).
Because Homer1a and Homer1c infusion appeared to produce opposite effects after behavioral despair, we further assessed the effects of AAV infusion after emotional reactivity by measuring locomotor hyperactivity in response to a novel environment. As illustrated in Figure 3c, both AAVs reversed the genotypic difference in the amount of novelty-induced locomotion expressed by the mice (Table 1). To assess for the effects of AAV infusion after the habituation of locomotor reactivity, we continued to assess the locomotor responsiveness of mice during four additional 30 min exposures to the locomotor activity chamber environment (Fig. 3d). In contrast to their similar effect on initial locomotor reactivity, only Homer1a infusion reversed the genotypic difference in locomotor habituation across repeated testing (Table 1), whereas Homer1c infusion appeared to enhance the size of the genotypic difference (Fig. 3d). Collectively, these data for emotional reactivity indicate that both Homer1a and Homer1c isoforms in the PFC regulate emotional reactivity to novelty, but only Homer1a plays an active role in the habituation of emotional responding. Moreover, these data provide novel support for a possible facilitatory role for Homer1c in certain forms of emotional reactivity.
AAV-Homer1c reverses genotypic differences in sensitivity to cocaine
In rats, an intra-accumbens infusion of AAV-Homer1c, but not AAV-Homer1a, reverses the sensitization of locomotor hyperactivity produced by cocaine, implicating an active role for Homer1c in regulating behavioral sensitivity to this stimulant (Szumlinski et al., 2005c). To examine the role for PFC Homer1a and Homer1c expression in regulating the “presensitized” cocaine behavioral phenotype of Homer1 KO mice (Szumlinski et al. 2004), we next assessed the effects of intra-PFC AAV infusion after cocaine-induced locomotion (0, 3, 10, and 30 mg/kg, i.p.). Whereas a genotypic difference in cocaine-induced locomotion was apparent in Homer1a mice (Fig. 4b), no difference in the dose–response function for cocaine-induced locomotion was observed between Homer1c WT and KO mice (Fig. 4c) (Table 1). Thus, consistent with our recent data for cocaine-sensitized rats (Szumlinski et al., 2005c), Homer1c isoforms actively regulate the behavioral-activating effects of cocaine in mice that are genetically “presensitized.”
Interestingly, the effect of AAV infusion after the locomotor response to saline (0 mg/kg cocaine) was opposite that observed for cocaine. Consistent with the data for locomotor habituation (Fig. 3d), Homer1a infusion reversed the genotypic difference in the locomotor response to saline (0 mg/kg cocaine) (Fig. 4b), whereas Homer1c infusion was without effect (Fig. 4c). Because these mice have been exposed repeatedly to the activity chambers before conducting this dose–response study, these data lend additional support for an active role for Homer1a in regulating the expression of emotional reactivity in a familiar environment.
AAV-Homer1c reverses genotypic differences in PFC glutamate content
In the cocaine sensitization model of schizophrenia in rats, Homer1c overexpression reverses the decline in basal extracellular levels of glutamate in the accumbens (Szumlinski et al., 2005c). Because the cocaine presensitized behavioral phenotype of Homer1 KO mice is associated with an elevation in PFC levels of glutamate (Szumlinski et al., 2005a), we assessed the effects of AAV infusion after basal extracellular glutamate content and glutamate clearance using the no net-flux in vivo microdialysis technique (Table 2). As illustrated in Figure 5, Homer1c selectively reversed the elevation in PFC basal glutamate content produced by Homer1 deletion, whereas Homer1a was without effect (Table 1). Consistent with our previous findings (Szumlinski et al., 2005a), statistical analyses revealed no effect of either gene deletion or AAV infusion on the slopes of the linear regressions in the present study, indicating no effect of manipulating Homer levels on glutamate clearance in the PFC (Table 2). Thus, the Homer1c isoform plays an active role in regulating basal glutamate content in the PFC without affecting glutamate clearance.
Differential effects of AAV-Homer1a and AAV-Homer1c infusion on the PFC glutamate response to cocaine
The cocaine “presensitized” behavioral phenotype of Homer1 KO mice is associated also with a blunted capacity of cocaine to elevate extracellular glutamate in the PFC, implicating a role for Homer isoforms in cocaine-induced glutamate release in this brain region (Szumlinski et al., 2005a). Because Homer1c overexpression reversed the effects of Homer1 deletion on both sensitivity to the locomotor effects of cocaine (Fig. 4c) and PFC basal glutamate content (Fig. 5), the effects of AAV infusion on the glutamate response to cocaine in the PFC were assessed using conventional in vivo microdialysis techniques. Consistent with the results of the no net-flux study, Homer1c infusion, but not Homer1a infusion, reversed the genotypic difference in basal extracellular glutamate (Table 2). Interestingly, cocaine failed to elevate PFC glutamate in either genotype infused with Homer1a (Fig. 6b). In contrast, cocaine elicited a rise in extracellular glutamate in Homer1c-KO mice and appeared to enhance the glutamate response to cocaine in their WT counterparts (Fig. 6c). Collectively, these data indicate that the Homer1c isoform actively regulates cocaine-stimulated glutamate release in the PFC and that Homer1a overexpression may inhibit evoked glutamate release in this brain region.
Discussion
Extensive behavioral and neurochemical phenotyping of Homer1 KO mice provided face, predictive, and construct validation of this mouse as a potential genetic animal model of schizophrenia (Szumlinski et al., 2004, 2005a). In mammals, alternative transcription of Homer1 yields both IEG and constitutively expressed gene products (Brakeman et al., 1997; Kato et al., 1998; Bottai et al., 2002). Using AAVs to distinguish between the IEG Homer1 isoform Homer1a and the constitutively expressed (CC) Homer1 isoform Homer1c, we found that PFC Homer1a overexpression selectively reversed genotypic differences observed for behavioral reactivity in paradigms involving repeated exposure to stressful stimuli. Conversely, Homer1c overexpression selectively reversed genotypic differences in working memory and sensorimotor processing, sensitivity to cocaine, and basal PFC glutamate content. Although, Homer1a overexpression failed to alter basal glutamate content, it prevented the cocaine-induced rise in PFC extracellular glutamate, whereas Homer1c overexpression facilitated cocaine-stimulated glutamate levels in KO mice and enhanced the increase in glutamate in WT animals. Collectively, these data indicate active and dissociable roles for both Homer1a and Homer1c in regulating excitatory neurotransmission within the PFC and demonstrate the importance of their relative expression in mediating normal cognitive, sensorimotor, and emotional processing as well as sensitivity to the psychomotor-activating effects of stimulant drugs.
Homer1c in cognitive and sensorimotor processing
Despite our understanding of the role for Homer1 isoforms in regulating neuronal morphology and cellular function (for reviews, see Xiao et al., 2000; Fagni et al., 2002; de Bartolomeis and Iasevoli, 2003), only recently have we begun to understand the role for Homer proteins in behavior. IEG Homer1 isoforms are induced in several limbo-corticostriatal structures by environmental stress/novelty (Vazdarjanova et al., 2002), footshock (Igaz et al., 2004), psychotomimetic drugs (Brakeman et al., 1997; Cochran et al., 2002; Nichols and Sanders-Bush, 2002; Fujiyama et al., 2003) and antipsychotic medications (de Bartolomeis et al., 2002; Polese et al., 2002), whereas Homer1b/c is downregulated in striatal structures by withdrawal from repeated cocaine (Swanson et al., 2001). These correlative data suggested an important role for the interplay between Homer1 isoforms in regulating behavior, in particular drug-induced changes in behavior. In support of this notion, Homer1 deletion or the intra-accumbens infusion of antisense oligonucleotides against Homer1 increases sensitivity to the acute psychomotor-activating effects of stimulants or dissociative anesthetics (Ghasemzadeh et al., 2003; Szumlinski et al., 2004, 2005a). Recent attempts to delineate the relative roles for IEG versus CC-Homer1 isoforms in behavior have indicated an important role for CC isoforms. Whereas an intrahippocampal infusion of AAV-Homer1a disrupts Morris water maze performance in wild-type rats, AAV-Homer1c infusion facilitates performance on this task (Klugmann et al., 2005). In conjunction with our virus reversal data for radial arm maze performance (Fig. 2), these data suggest a necessary and facilitatory role for the Homer1c isoform in mediating neural plasticity within cortical structures associated with normal learning and memory processes. Because AAV-Homer1c infusion selectively reversed the sensorimotor gating deficits noted for KO mice, it is suggested that an improvement in attentional processing by restoration of Homer1c expression in the PFC of KO mice may also have contributed to the observed rescue of radial arm maze performance.
Homer1a and coping with stress
Whereas AAV-Homer1a infusion into the PFC did not influence radial arm maze performance or prepulse inhibition of acoustic startle, AAV-Homer1a selectively reversed the effects of Homer1 deletion on swimming behavior in a Porsolt swim test, locomotor habituation, and the locomotor response to a saline injection. It is noteworthy that all of the paradigms in which AAV-Homer1a was selective in its reversal of the KO phenotype involved the retention of information regarding a stressful or aversive event and its subsequent recall within a 24 h period. IEG Homer1 isoforms are upregulated in cortical structures, including the frontal cortex, by exposure to novel environments (Vazdarjanova et al., 2002) and by the administration of psychotomimetic drugs (Brakeman et al., 1997; Cochran et al., 2002; Nichols and Sanders-Bush, 2002; Fujiyama et al., 2003). More recently, an upregulation of Homer1a was observed in the hippocampus at 24 h after a one-trial avoidance learning task, indicating that this IEG exhibits a protracted profile of expression that may impact responding to a subsequent aversive event (Igaz et al., 2004). Together, these data implicate mutations affecting the induction of IEG Homer1 transcriptional variants as a genetic determinant of vulnerability to stress. Moreover, it is suggested from these data that the induction of Homer1a in cortical and allocortical structures is a necessary cellular response to stress that may enable an organism to make an adaptive behavioral response in the short term.
Homer isoforms, PFC glutamate, and schizophrenia
A dysfunction in limbo-corticostriatal glutamate is implicated in the pathophysiology of schizophrenia, a neuropsychiatric condition characterized by abnormalities in cognitive, motivational, sensorimotor, and emotional processing (Lewis and Lieberman, 2000; Tsai and Coyle, 2002; Krystal et al., 2003). In humans, Homer1 (5q14.2) is localized to chromosome 5 (Xiao et al., 1998), and a number of linkage studies indicate susceptibility loci on this chromosome in individuals affected with schizophrenia or schizoaffective disorder (Levinson et al., 2000; Gurling et al., 2001; Paunio et al., 2001). A IVS4 + A>G substitution in intron 4 of Homer1 has been associated with schizophrenia in a large but very heterogeneous population (Norton et al., 2003). The N-terminal EVH1 domain of Homer1 is encoded by exons 1–5, whereas the leucine zipper/CC-domain is encoded by exons 6–10 (Bottai et al., 2002; Norton et al., 2003). A large central intron 5 separates these two encoding regions and contains the sequences involved in the activity-dependent premature termination of Homer1 transcription (Bottai et al., 2002). Thus, if substantiated by further linkage analyses, a Homer1 IVS4 + A>G substitution would be expected to impact both IEG and CC-Homer1 transcription, thereby implicating both IEG and CC-Homer1 isoforms in the pathophysiology of schizophrenia.
Deletion of Homer1, but not of Homer2, produces abnormalities in PFC glutamate that are hypothesized to underlie the selective detrimental effect of Homer1 deletion on cognitive, sensorimotor, emotional, and motivational processing (Szumlinski et al., 2004, 2005a). In support of this link between PFC glutamate and behavior, amelioration of the working memory and sensorimotor deficits of KO mice by PFC Homer1c overexpression were accompanied by a reduction in basal extracellular glutamate content and a facilitation of stimulated glutamate release. Thus, by restoring normal (WT-type) glutamate tone within the PFC, Homer1c may reduce the autoinhibition of glutamatergic transmission, thus enabling the gating of relevant sensory information and facilitating attentional processes required for complex learning tasks. Because PFC Homer1a expression did not influence the phenotype of KO mice along variables associated with working memory, sensorimotor function or PFC glutamate implicates mutations affecting the transcription of CC-Homer1 isoforms or the ability of CC-Homers to multimerize, rather than mutations affecting EVH1 binding, in the manifestation of dysregulated excitatory transmission within the PFC underlying cognitive and sensorimotor gating dysfunction.
Mutational analyses have associated a mutation in GRM5 with schizophrenia (Devon et al., 2001), and mice with deletions of either mGluR1 or mGluR5 exhibit prepulse inhibition deficits akin to Homer1 KO mice (Brody et al., 2003, 2004). In rodent models, antagonists at mGluR5 potentiate the deficits in prepulse inhibition produced by NMDA receptor blockade (Henry et al., 2002; Pietraszek et al., 2005), whereas group 1 mGluR agonists can reverse these deficits (Maeda et al., 2003). Because deletion of genes encoding CC-Homers downregulates group1 mGluR function in all cortical areas (Szumlinski et al., 2004) and group1 mGluRs regulate extracellular levels of glutamate in the PFC (Melendez et al., 2005), it is suggested further that the formation of CC-Homer-group1 mGluR signaling complexes may be essential for maintaining basal extracellular glutamate levels in the PFC and for regulating glutamate release during attentional or cognitive tasks.
CC-Homers and cocaine-induced neural plasticity
Epidemiological studies have revealed a high degree of comorbidity between schizophrenia and psychomotor stimulant abuse disorders (Batel, 2000; Gerber and Tonegawa, 2004). Consistent with this, Homer1 KO mice exhibit increased sensitivity to both the locomotor-activating and the conditioned rewarding effects of cocaine (Szumlinski et al., 2004). In support of a more critical role for CC-Homer isoforms in the regulation of behavioral sensitivity to cocaine, deletion of Homer2, a gene with no known IEG transcriptional variants, also enhances cocaine behavioral and neurochemical sensitivity, which is reversed by an intra-accumbens infusion of AAV-Homer2b (Szumlinski et al., 2004). Moreover, an intra-accumbens infusion of a TAT-Homer2a fusion protein or AAVs carrying Homer1c and Homer2b, but not Homer1a, completely prevented the expression of cocaine-induced behavioral and glutamate sensitization in rats (Szumlinski et al., 2005c). The present results extend these data to the PFC and indicate an inhibitory role for corticoaccumbens CC-Homer1 isoforms in sensitivity to psychostimulants. PFC Homer1c expression also facilitated the capacity of cocaine to enhance extracellular glutamate in the PFC, a finding consistent with neuroimaging evidence demonstrating an inhibitory role for frontal cortical structures, in particular the orbitofrontal cortex, in gating behavioral responses to cocaine and cocaine-associated stimuli (Goldstein and Volkow, 2002). Thus, it may be proposed that by maintaining glutamate tone in the frontal cortex, Homer1c also enables cortical activation inhibiting addiction-related behaviors.
Conclusions
Through an examination of the effects of restoring Homer1a and Homer1c expression to the PFC of Homer1 KO mice, the present study implicates a distinct and active role for the Homer1a isoform in regulating the ability to cope with repeated stress and for the Homer1c isoform in the regulation of basal and stimulated glutamate release in the PFC mediating attentional and cognitive processing. Although the cellular mechanisms underlying the distinctions between Homer1a and Homer1c isoforms in regulating behavior associated with corticoaccumbens glutamate transmission are not defined in the present study, these data support Homer1 polymorphisms affecting the transcription of IEG and CC-Homer1 isoforms in the etiology of schizophrenia, as well as stimulant abuse comorbidity. Moreover, these data implicate Homer-associated intracellular signaling mechanisms as potential targets for the development of novel pharmacotherapeutics for the treatment of these disorders.
Footnotes
This work was supported in part by United States Public Health Service Grants DA-11742 and DA-10307 (P.F.W.) and DA-03906, DA-14185, and MH-40817 (P.W.K.) and a National Alliance for Research on Schizophrenia and Depression Young Investigator Award to K.K.S. We thank Stephen Welt for his technical assistance, Joe Vallone for genotyping and maintenance of the mouse colony, and Shin Kang and Marlin Dehoff for generating the KO mice and assays for genotyping.
Correspondence should be addressed to Dr. Karen K. Szumlinski, Department of Psychology, University of California, Santa Barbara, Santa Barbara, CA 93106-9660. E-mail: szumlinski{at}psych.ucsb.edu.
K. D. Lominac's present address: Department of Psychology, University of California, Santa Barbara, Santa Barbara, CA 93106-9660.
M. Klugmann's present address: Department of Neurobiology, Interdisciplinary Center for Neurosciences, University of Heidelberg, Im Neuenheimer Feld 364, D-69129 Heidelberg, Germany.
DOI:10.1523/JNEUROSCI.3764-05.2005
Copyright © 2005 Society for Neuroscience 0270-6474/05/2511586-09$15.00/0