Abstract
Noradrenergic (NE) α1-adrenoceptors (α1-ARs) contribute to arousal mechanisms and play an important role in therapeutic medications such as those for the treatment of posttraumatic stress disorder (PTSD). However, little is known about how α1-AR stimulation influences neuronal firing in the dorsolateral prefrontal cortex (dlPFC), a newly evolved region that is dysfunctional in PTSD and other mental illnesses. The current study examined the effects of α1-AR manipulation on neuronal firing in dlPFC of rhesus monkeys performing a visuospatial working memory task, focusing on the “delay cells” that maintain spatially tuned information across the delay period. Iontophoresis of the α1-AR antagonist HEAT (2-{[β-(4-hydroxyphenyl)ethyl]aminomethyl}-1-tetralone) had mixed effects, reducing firing in a majority of neurons but having nonsignificant excitatory effects or no effect in remaining delay cells. These data suggest that endogenous NE has excitatory effects in some delay cells under basal conditions. In contrast, the α1-AR agonists phenylephrine and cirazoline suppressed delay cell firing and this was blocked by coadministration of HEAT. These results indicate an inverted-U dose response for α1-AR actions, with mixed excitatory actions under basal conditions and suppressed firing with high levels of α1-AR stimulation such as with stress exposure. Immunoelectron microscopy revealed α1-AR expression presynaptically in axons and axon terminals and postsynaptically in spines, dendrites, and astrocytes. It is possible that α1-AR excitatory effects arise from presynaptic excitation of glutamate release, whereas postsynaptic actions suppress firing through calcium–protein kinase C opening of potassium channels on spines. The latter may predominate under stressful conditions, leading to loss of dlPFC regulation during uncontrollable stress.
SIGNIFICANCE STATEMENT Noradrenergic stimulation of α1-adrenoceptors (α1-ARs) is implicated in posttraumatic stress disorder (PTSD) and other mental disorders that involve dysfunction of the prefrontal cortex, a brain region that provides top-down control. However, the location and contribution of α1-ARs to prefrontal cortical physiology in primates has received little attention. This study found that α1-ARs are located near prefrontal synapses and that α1-AR stimulation has mixed effects under basal conditions. However, high levels of α1-AR stimulation, as occur with stress, suppress neuronal firing. These findings help to explain why we lose top-down control under conditions of uncontrollable stress when there are high levels of noradrenergic release in brain and why blocking α1-AR, such as with prazosin, may be helpful in the treatment of PTSD.
- alpha-1 receptor
- dorsolateral prefrontal cortex
- mental disorders
- noradrenergic receptor
- PTSD
- working memory
Introduction
It has been long appreciated that noradrenergic (NE) stimulation of α1-adrenoceptors (α1-ARs) contributes to cortical arousal (Waterhouse et al., 1981; McCormick et al., 1991; Berridge and España, 2000). NE has varied interactions with adrenergic receptors, with high affinity for the α1d-AR and α2A-AR subtypes, but low affinity for the α1A-AR and β-AR subtypes (Horie et al., 1995; Arnsten, 2000). Therefore, differing receptor mechanisms become engaged with increasing NE release; low to none during sleep, moderate levels in the alert, nonstressed state, and high levels of release during psychological or physiological stress (Goldstein et al., 1996; Kobori et al., 2006). α1-AR actions are especially evident under stressful conditions of high NE release, when they strengthen the affective functioning of the amygdala (Ferry et al., 1999; Rajbhandari et al., 2015) but weaken the cognitive abilities of the prefrontal cortex (PFC) (Birnbaum et al., 1999). Therefore, they are particularly relevant to disorders such as posttraumatic stress disorder (PTSD).
Treatment of PFC disorders often involves medications that have α1-AR-blocking actions. For example, PTSD symptoms involve impaired PFC regulation of emotion (Fitzgerald et al., 2018) and the α1-AR antagonist prazosin is in widespread use for the treatment of PTSD in veterans, active duty soldiers, and civilians (for review, see Arnsten et al., 2015). α1-AR blockade is also a common characteristic of atypical antipsychotic drug actions (Baldessarini et al., 1992; Bymaster et al., 1996) used to treat mental disorders that involve dysfunction of the dorsolateral PFC (dlPFC; Glantz and Lewis, 2000). However, despite this therapeutic relevance, relatively little is known about the cellular actions of α1-ARs in primate dlPFC.
The primate dlPFC contains the neuronal microcircuits needed for visuospatial working memory, with “delay cells” that maintain spatially tuned firing across the delay period (Goldman-Rakic, 1995). This persistent firing arises from recurrent excitatory connections between layer III pyramidal cells (Kritzer and Goldman-Rakic, 1995) and relies on NMDAR synapses on spines (Wang et al., 2013b; Ma et al., 2015). Cognitive data initially suggested that NE α1-ARs had little influence on dlPFC working memory function because infusions of the α1-AR antagonist prazosin into the monkey dlPFC had no effect on working memory performance (Li and Mei, 1994). However, later research showed that infusion of the α1-AR agonist phenylephrine (PE) produced a marked, delay-related impairment in performance (Mao et al., 1999) similar to that seen in rat mPFC (Arnsten and Jentsch, 1997). Because stress-induced cognitive deficits could be blocked by α1-AR antagonist infusion in rat mPFC (Birnbaum et al., 1999), the data suggested that α1-AR actions were only engaged in PFC when high levels of NE release were induced by stress exposure and that they suppressed dlPFC neuronal firing and function via activation of calcium–protein kinase C (PKC) signaling (Birnbaum et al., 2004). In contrast, in vitro physiological and behavioral studies in rats indicated that NE α1-ARs can have excitatory actions in mPFC, for example, via increasing glutamate release (Zhang et al., 2013), and contributes to cognitive improvement (Puumala et al., 1997; Lapiz and Morilak, 2006; Berridge et al., 2012; Hvoslef-Eide et al., 2015), suggesting apparent species differences. However, α1-AR cellular actions in monkey dlPFC have received minimal examination and thus may be more similar to rat than presently thought.
The current study provides the first comprehensive examination of α1-AR cellular actions in monkey dlPFC. We examined the effects of local iontophoresis of α1-AR agents directly onto dlPFC neurons in monkeys performing a visuospatial working memory task, assessing the α1-AR antagonist HEAT (2-{[β-(4-hydroxyphenyl)ethyl]aminomethyl}-1-tetralone) to reveal the influence of endogenous NE α1-AR actions, as well as two α1-AR agonists, PE and cirazoline. We also performed immunoelectron microscopy (immuno-EM) of layer III monkey dlPFC to determine the ultrastructural localization of α1-ARs and how they compare to rat mPFC, in which α1-ARs have been found both presynaptically and postsynaptically (Mitrano et al., 2014). We found the first physiological evidence of an α1-AR inverted-U dose response, where presynaptic versus postsynaptic effects may promote excitatory versus suppressive actions on dlPFC delay cell firing.
Materials and Methods
Physiological recordings in cognitively engaged monkeys
Subjects.
Two male, adult rhesus monkeys (Macaca mulatta) were used in the current study and were cared for under the guidelines of the National Institutes of Health (NIH) and the Yale Institutional Animal Care and Use Committee (IACUC).
Oculomotor delayed response (ODR) task.
The monkeys were seated in primate chairs with their heads fixed and faced a 27-inch computer monitor 30 inches in front of them. The monkeys' eye positions were monitored with the ISCAN Eye Movement Monitoring System. The monkeys were pretrained on the visuospatial ODR task, which required the subject to make a memory-guided saccade to a remembered visuospatial target. Patients with schizophrenia have been shown to be impaired on a human version of this task (Keedy et al., 2006). The ODR task was generated by the PICTO system (custom-designed, Windows-based data acquisition software). The task is illustrated in Figure 1A. A central small white circle was illuminated on the computer monitor, which served as the fixation target. To initiate a trial, the animal fixated this central target and maintained fixation for 0.5 s (fixation period), whereupon a cue (the same sized white circle) was illuminated for a period of 0.5 s (cue period) at 1 of 8 peripheral targets located at an eccentricity of 13° with respect to the fixation spot. After the cue was extinguished, a 2.5 s delay period followed. The subject was required to maintain central fixation throughout both the cue presentation and the delay period. At the end of the delay, the fixation spot was extinguished, which instructed the monkey to make a memory-guided saccade to the location where the cue had been shown before the delay period. A trial was considered successful if the subject's response was completed within 0.5 s of the offset of the fixation spot and was within 2° around the correct cue location. The subject was rewarded with fruit juice immediately after every successful response. The position of the stimulus was randomized over trials such that it had to be remembered on a trial-by-trial basis. The intertrial intervals (ITIs) were at least 3 s. The subject performed 1000–1500 trials per session.
Recording locus.
Before recording, the animals underwent a magnetic resonance image (MRI) scan to aid in the placement of a recording chamber over the caudal principal sulcus (as illustrated in Fig. 1B) and to later guide electrophysiological recordings. MRI-compatible materials were used for the implant so that a second MRI could be performed after implantation to confirm recording chamber position.
In vivo single-unit recordings and iontophoresis.
Iontophoresis was used to apply the α1-AR agonists PE and cirazoline and/or the α1-AR antagonist HEAT hydrochloride, near dlPFC neurons. PE, cirazoline, and HEAT hydrochloride were from Tocris Bioscience. Drug solutions (5–10 mm, pH = 3–4) were made using sterilized water. Iontophoretic electrodes were constructed with a 20-μm-pitch carbon fiber (ELSI) inserted in the central barrel of a seven-barrel nonfilamented capillary glass (Friedrich and Dimmock). The assembly was pulled using a multipipette electrode puller (PMP-107L; Microdata Instrument) and the tip was beveled to obtain the finished electrode. Finished electrodes had impedances of 0.3–1.5 MΩ (at 1 kHz) and tip sizes of 30–40 μm. The outer barrels of the electrode were then filled with three drug solutions (two consecutive barrels each) and the solutions were pushed to the tip of the electrode using compressed air. A Neurophore BH2 iontophoretic system (Medical Systems) was used to control the delivery of the drugs. The drug was ejected at currents that varied from 10 to 100 nA. Retaining currents of −5 to −10 nA were used in a cycled manner (1 s on, 1 s off) when not applying drugs. Drug ejection did not create noise in the recording and there was no systematic change in either spike amplitude or time course at any ejection current.
The electrode was mounted on a MO-95 micromanipulator (Narishige) in a 25 gauge stainless steel guide tube. The dura mater was punctured using the guide tube to facilitate access of the electrode into the cortex. Extracellular voltage was amplified using a AC/DC differential preamplifier (Model 3000; A-M Systems) and band-pass filtered (180 Hz to 6 kHz, 20 dB gain, 4-pole Butterworth; Kron-Hite). Signals were digitized (15 kHz, micro 1401; Cambridge Electronics Design) and acquired using the Spike2 software (Cambridge Electronics Design). Neuronal activity was analyzed using waveform sorting by a template-matching algorithm. Poststimulus time histograms (PSTHs) and rasters were constructed online to determine the relationship of unit activity to the task. Unit activity was measured in spikes per second. We classified four different kinds of ODR task-related cells: fixation cells, cue cells, delay cells, and response cells. If the rasters showed that a neuron displayed task-related activity, then recording continued and pharmacological testing was performed. A total of 54 delay cells were recorded and tested with agents (32 from Monkey H, 22 from Monkey J).
Neuronal activities were first collected from the cell under a control condition in which at least eight trials at each of eight cue locations were obtained. A typical delay cell is shown in Figure 1C. Upon establishing the stability of the cells' activity, this control condition was followed by iontophoretic application of drug(s). Dose-dependent effects of the drug were tested in two or more consecutive conditions, followed by a recovery condition or a reversal condition. Each condition had approximately eight (range, six to 12) trials at each location to allow for statistical analyses of drug effects. Recovery conditions are especially important for treatments that reduce firing to demonstrate that the neuron is still healthy. However, given the prolonged effects of many compounds, such as through second messenger actions, a statistically significant but not complete restoration to baseline was required. Drugs were continuously applied at a relevant current throughout a given condition. Please note that the term “dose” signifies “iontophoretic dose” herein because the amount of drug released is proportional to the ejection current. However, it is impossible to know the exact amount of drug released and the extent of drug dispersal into brain tissue because this varies based on many factors including the lipophilicity of the compound and the exact placement of the electrode in regard to nearby white matter. However, it is the only method that allows application of drug without disturbing the position of the underlying neuron because there is no fluid ejection to move the neuron away from the electrode. Finally, it is common for the arousal neuromodulators such as NE and dopamine (DA) to produce an inverted-U dose response on dlPFC cell firing. For example, DA stimulation of D1R has an inverted-U dose response, in which both D1R antagonists and D1R agonists suppress firing (Williams and Goldman-Rakic, 1995; Vijayraghavan et al., 2007). This is especially common when animals are working under optimal arousal conditions, as in our studies.
Data analyses.
Each trial in the ODR task was divided into four epochs: initial fixation, cue, delay and response (saccade). The initial fixation epoch lasted for 0.5 s. The cue epoch lasted for 0.5 s and corresponds to the stimulus presentation phase of the task. The delay lasted for 2.5 s and reflects the mnemonic component of the task. The response phase started immediately after the delay epoch and lasted ∼1.5 s. Data analysis was performed in MATLAB and SPSS. Spike density functions were constructed in 50 ms windows. Two-way ANOVA was used to examine the spatial tuned task-related activity with regard to different periods of the task (fixation, cue, delay, and response vs ITI) and different cue locations. This study focused on delay cells that represent working memory. Many delay cells fire during the cue and/or response epochs as well as the delay epoch; given their variable responses to the cue and response epochs, data analyses focused on the delay epoch using the entire delay period (0–2.5 s). One-way ANOVA or t test were used to assess the effects of drug application on task-related activity. In the interest of brevity, figures often show the neurons' preferred direction compared with just one nonpreferred direction, the “anti-preferred” direction directly opposed to the neurons' preferred direction. For delay cells, spatial tuning was assessed by comparing firing levels for the neuron's preferred direction vs its nonpreferred directions. Quantification of spatial tuning was performed by calculating a measure of d′ using the following formula:
Electron microscopy
Tissue processing.
Fixed, long-term-stored rhesus monkey brain tissue from one female monkey (age of 18 years) was used for this study. In general, brain tissues were prepared for storage in our brain bank as follows, with euthanasia performed in accordance with the guidelines of the Yale IACUC and NIH's Guidelines for the Care and Use of Experimental Animals. Monkeys were deeply anesthetized before transcardial perfusion of artificial CSF, followed by 4% paraformaldehyde/0.05% glutaraldehyde in 100 mm PBS. Following perfusion, a craniotomy was performed and the entire brain was removed and dissected, including a frontal block containing the primary region of interest surrounding the principal sulcus. The brains were sectioned coronally at 60 μm on a vibratome (Leica) across the entire rostrocaudal extent of the dlPFC (Walker's area 46). The sections were cryoprotected through increasing concentrations of sucrose solution (10%, 20%, and 30% each for 2 h and then 30% overnight), cooled rapidly using liquid nitrogen, and stored at −80°C.
Histology and immunoreagents.
The antibody used to detect α1-AR in the current study (PA1–047; Thermo Scientific) is the same one used by the Weinshenker group (Mitrano et al., 2012, 2014) to localize α1-ARs in rat PFC. This rabbit polyclonal antibody has been extensively characterized and was raised against a synthetic peptide corresponding to residues K(339)FSREKKAKT(349) of the third intracellular loop of human α1-AR. It has been previously shown to recognize α1-ARs with a high degree of specificity in different tissues and cell types by Western blot and immunohistochemistry procedures (Nakadate et al., 2001; Wee et al., 2008; Cognato et al., 2010; Rajbhandari et al., 2015). We further validated specificity by performing a peptide adsorption control experiment using the PA1–047 immunizing peptide (Thermo Fisher Scientific, catalog #PEP-216) in which no positive labeling was observed. Normal sera and IgG-free BSA were from Jackson Immunoresearch.
Pre-embedding peroxidase immunocytochemistry.
Sections of dlPFC were processed for α1-AR immunocytochemistry. To enable penetration of immunoreagents, all sections went through three freeze–thaw cycles in liquid nitrogen. Nonspecific reactivity was suppressed with 10% NGS and 2% BSA and antibody penetration was enhanced with 0.3% Triton X-100 in 50 mm TBS. As described previously (Paspalas et al., 2013), dlPFC sections were incubated for 72 h at 4°C with α1-AR antibody in TBS, and transferred for 2 h at room temperature to species-specific biotinylated Fab′ or F(ab′)2 fragments in TBS. Sections were incubated with the avidin-biotin peroxidase complex (ABC) (1:300; Vector Laboratories) and then visualized in 0.025% 3,3-diaminobenzidine tetrahydrochloride (DAB; Sigma-Aldrich) as a chromogen in 100 mm PB with the addition of 0.005% hydrogen peroxide for 10 min. After the DAB reaction, sections were exposed to osmification, dehydration, and epoxy resin embedding on microscope slides. Omission of α1-AR primary antibody or substitution with nonimmune rabbit serum resulted in a complete lack of immunoperoxidase labeling.
Electron microscopy and data analysis.
All sections were processed as described previously (Paspalas et al., 2013). Briefly, blocks containing dlPFC layer III were dissected and mounted onto resin blocks. The specimens were cut into 50 nm sections using an ultramicrotome (Leica) and analyzed under a JEM1010 transmission electron microscope (Jeol) at 80 kV. Several plastic blocks of each brain were examined using the fourth to 12th surface-most sections of each block (i.e., 200–600 nm) to sample the superficial component of sections, avoiding penetration artifacts. Structures were digitally captured at 25,000× to 100,000× magnification with a Bioscan camera and individual panels were adjusted for brightness and contrast using Adobe Photoshop and Illustrator CC.2017.01 image editing software. Approximately 300 micrographs of selected areas of neuropil with immunopositive profiles were used for analyses. For profile identification, we adopted the criteria summarized by Peters et al. (1991). Dendritic spines in the PFC are typically long and thin, devoid of mitochondria, and contain a noticeable postsynaptic density at asymmetric synapses. Dendritic shafts were typically round in perpendicular planes or elongated when assessed in horizontal planes, usually containing mitochondria and numerous tubular and pleomorphic cellular organelles. Numerous dendritic shafts received synaptic inputs. Axon terminals contained accumulations of synaptic vesicles and the axoplasm of these terminals usually contained neurofilaments and mitochondria. The synaptic innervations made by these axon terminals were either asymmetric, containing spherical vesicles, or symmetric, containing pleomorphic vesicles, with thick or thin postsynaptic density, respectively. Unmyelinated axons were small, round processes with a predominantly even and regular shape, traversing the neuropil in a straight orientation, often forming bundles in perpendicular planes. Astroglial processes were typically of irregular morphology and filled the space around neuronal elements.
Results
Physiology
Effects of blocking endogenous NE stimulation of α1-AR with the antagonist HEAT
We first examined the effect of blocking α1-AR on the delay-related firing in dlPFC delay cells to assess the role of endogenous NE stimulation α1-AR on working memory function in awake behaving animals. Although behavioral studies had found little influence on working memory performance when an α1-AR antagonist was infused into the dlPFC (Li and Mei, 1994), it is not known how local α1-AR blockade would effect dlPFC neuronal firing. Here, we trained two monkeys to perform an ODR spatial working memory task while recording from delay cells in area 46 of dlPFC (Fig. 1). The α1-AR antagonist HEAT hydrochloride was delivered via iontophoresis near the neurons under investigation. In this experiment, 14 neurons with delay-related mnemonic activity were isolated and tested with HEAT. We found that HEAT had a mixed effect on PFC delay-related firing. As shown in Figure 2A, HEAT caused a significant decrease in delay-related firing in 8 of 14 delay cells, consistent with endogenous NE having excitatory actions under basal conditions. However, HEAT produced either a nonsignificant increase in firing or no change in firing in the remaining six delay cells. A single-cell example of HEAT decreasing firing is shown in Figure 2B (control vs HEAT at 15 nA, p = 0.005, t(11) = 4.87, unpaired t test) and a single-cell example of its tendency to increase firing in another neuron is shown in Figure 2C (control vs HEAT at 15 nA, p = 0.35, t(12) = 0.96, unpaired t test). Overall, when the 14 neurons were combined, HEAT produced a significant reduction in firing (p = 0.047, t(13) = 2.195, two-tailed paired t test).
Effects of stimulating α1-ARs with the agonists PE and cirazoline
In contrast to the mixed effects of HEAT, stimulation of α1-ARs with agonists had a more consistent effect, suppressing neuronal firing. We tested two α1-AR agonists, PE and cirazoline, over a wide range of doses. Our previous work had examined only very high-dose PE administration (Birnbaum et al., 2004), so a more comprehensive characterization was needed. A total of 30 delay cells were tested with PE (25–50 nA) and PE was found to significantly suppress firing in 27 of the 30 cells. As illustrated in Figure 3A, iontophoretic application of PE produced a dose-related reduction in delay-related firing. PE at dose of 25 nA significantly reduced the delay activity for the neuron's preferred direction (control vs PE at 25 nA, p = 0.026, t(15) = 2.474, unpaired t test), but not for its nonpreferred direction (control vs PE at 25 nA, p = 0.66, t(15) = 0.43, unpaired t test), whereas a higher dose of PE (40 nA) further suppressed delay firing for the neuron's preferred direction (control vs PE at 25 nA, p = 0.036, t(11) = 2.38, unpaired t test). The suppressing effects of PE were consistent at the population level. Figure 3B shows the averaged effect of PE on delay cell firing in all 30 delay cells tested. Statistical analysis showed that PE application significantly reduced neuronal firing during the delay epoch for the neurons' preferred direction (Fig. 3C; p = 0.0003, t(30) = 4.087, two-tailed paired t test), but not the nonpreferred direction (Fig. 3C; p = 0.12, t(30) = 1.588, two-tailed paired t test) for all 30 delay cells. To confirm that PE reduced direction selectivity, we calculated a measure of d′ to determine whether PE eroded the spatial tuning of delay cells. This measure captures how well a delay cell can represent a spatial position over the delay epoch in the absence of sensory stimulation and thus is particularly important to the strength of working memory. A greater d′ value indicates greater directional selectivity, that is, greater spatial tuning. A total of 26 of 30 delay cells showed significantly lower d′ in the PE condition compared with the control condition (Fig. 3D; p < 0.0001, t(30) = 5.544, two-tailed paired t test).
PE excited delay-related firing in three of the 30 delay cells at the same doses that suppressed firing in the majority of neurons. An example of PE's excitatory effect is shown in Figure 4. In this case, PE significantly enhanced the delay activity for the neuron's preferred direction (control vs PE at 40 nA, p = 0.049, t(14) = 2.156, unpaired t test), but not for its nonpreferred direction (control vs PE at 40 nA, p = 0.095, t(15) = 1.779, unpaired t test) and increased the spatial tuning (2.3 for the control condition to 3.1 for the PE condition). However, these enhancing effects were remarkably rare.
The second α1-AR agonist cirazoline consistently suppressed delay-related firing in 10 of 10 delay cells tested. As shown in Figure 5A, cirazoline produced a dose-related reduction in delay-related firing, with marked suppression following 40 nA (control vs cirazoline at 25 nA, p = 0.02, t(12) = 2.659, unpaired t test; control vs cirazoline at 40 nA, p < 0.0001, t(15) = 9.884, unpaired t test). Delay-related firing returned toward control levels when the drug was no longer applied (recovery condition; recovery vs cirazoline at 40 nA, p < 0.0001, t(17) = 5.128, unpaired t test). The suppressing effects of cirazoline were highly consistent at the population level (Fig. 5B). Statistical analysis showed that cirazoline significantly reduced neuronal firing during the delay epoch for the neurons' preferred direction (Fig. 5C; p = 0.001, t(11) = 4.313, two-tailed paired t test), but not for the nonpreferred direction (Fig. 5C; p = 0.194, t(11) = 1.382, two-tailed paired t test). Similar to the effects of PE, iontophoresis of cirazoline significantly reduced the spatial tuning of delay cell firing during the delay epoch by decreasing d′ (Fig. 5D; p = 0.002, t(11) = 4.052, two-tailed paired t test).
Challenging the PE response with HEAT
To test whether the suppressing effects of PE occurred through actions at α1-ARs, we coapplied the α1-AR antagonist HEAT with PE and found that HEAT could prevent the suppressing effects of PE. As shown in Figure 6A, HEAT at a low dose of 10 nA produced a slight, nonsignificant reduction in delay-related firing for the neuron's preferred direction. When PE was then coapplied with HEAT, firing was unchanged. However, when HEAT was no longer applied, PE markedly suppressed delay-related firing. HEAT's ability to block the suppressive effects of PE action was established in 5/5 neurons (Fig. 6B; overall: p = 0.85, Friedman test; pairwise comparisons: control vs HEAT: p = 0.81; HEAT vs HEAT+PE: p = 0.81, Wilcoxon signed-rank test). These results are consistent with PE acting at α1-AR.
Immuno-EM
α1-AR distribution in presynaptic and postsynaptic neuronal compartments in monkey dlPFC
We found significant α1-AR labeling in both presynaptic and postsynaptic neuronal compartments in primate layer III dlPFC (Fig. 7). Presynaptic α1-AR labeling could be seen in both asymmetric axon terminals and unmyelinated axons. Within axon terminals, α1-AR were often found perisynaptically or extrasynaptically bordering the excitatory synapse along the axolemma, but were not observed within the active zone per se (Fig. 7A). In addition to the terminal distribution, α1-ARs in axons were also observed in preterminal axons or intervaricose segments traversing through the neuropil, which may represent receptors trafficking to the terminal (data not shown).
The predominant postsynaptic localization of α1-AR was in dendritic spines (Fig. 7B). Labeling was especially evident on spines with long, thin necks, a prominent postsynaptic density (PSD), and a spine apparatus (the extension of the calcium-storing, smooth endoplasmic reticulum into the spine), all common features in primate layer III dlPFC. The labeling was often observed extrasynaptically or perisynaptically to glutamatergic-like axospinous asymmetric synapses, usually in close proximity to the spine apparatus (Fig. 7B). Expression of α1-ARs was not detected in spines receiving symmetric synapses. Dendritic shafts also expressed α1-AR immunoreactivity at nonsynaptic membranes, as well as within the intracellular compartment, possibly being trafficked along microtubules to distal sections of the neuron (data not shown).
Localization of α1-ARs in astrocytic processes in monkey dlPFC layer III
α1-ARs were also prominent in dlPFC layer III astrocytes (Fig. 8). The labeling pattern was targeted to perisynaptic astrocytic processes ensheathing axospinous synapses (Fig. 8A,B). Occasionally, α1-AR-labeled astrocytic processes near axodendritic glutamatergic-like synapses in close proximity to dendritic mitochondria (Fig. 8C). The labeling pattern was most pronounced along the astroglial plasma membrane and not diffusely distributed throughout the cytoplasm.
In summary, α1-ARs were observed in both presynaptic and postsynaptic compartments of neurons, as well as in astroglial processes.
Discussion
The current data show the first evidence of an NE α1-AR inverted-U dose response on delay cell firing in the primate dlPFC and the first ultrastructural data showing α1-AR expression presynaptically on axon terminals and postsynaptically on layer III spines (schematically illustrated in Fig. 9). The physiological recordings revealed excitatory effects of endogenous NE at α1-AR under nonstress conditions in a large subset of neurons because there was reduced neuronal firing when these actions were blocked by HEAT. Low doses of the α1-AR agonist PE also produced excitatory effects in a small number of neurons, suggesting that the prevalent α1-AR response under basal NE, nonstress conditions is excitatory (Fig. 9). In contrast, iontophoresis of higher doses of PE and cirazoline predominately suppressed firing and this reduction was reversed by co-iontophoresis with HEAT, consistent with α1-AR drug actions. The suppression of firing with high levels of α1-AR stimulation likely occurs during stress exposure (Fig. 9) when there are very high levels of NE release in PFC (Nakane et al., 1994; Finlay et al., 1995) and dlPFC function is rapidly impaired (Arnsten, 2009).
Immuno-EM showed that the predominant location of α1-AR is on spines, suggesting that drugs acted directly on pyramidal cells. However, given the architecture of dlPFC microcircuits, reduced pyramidal cell firing would also decrease interneuron firing. Waveform analyses are inadequate to definitively characterize cell recordings as pyramidal cells or interneurons, but the data suggest an overall reduction in dlPFC microcircuit activity following α1-AR stimulation.
The excitatory actions of endogenous, basal levels of NE versus the suppressive effects of cirazoline may arise from their differing affinity for α1-AR subtypes. NE has much higher affinity for α1d-AR (42 nm) than for α1a-AR (990 nm), whereas cirazoline has the opposite profile (α1a-AR: 120 nm vs α1d-AR: 660 nm; Horie et al., 1995). In contrast, PE is nonselective between subtypes (Minneman et al., 1994). It is possible that α1-AR excitatory effects in primate dlPFC involve α1d-AR actions, for example, increasing presynaptic glutamate release, whereas the suppressive effects involve α1a-AR actions, for example, at postsynaptic receptors that engage calcium-PKC-K+ signaling (Datta and Arnsten, 2018). The latter idea is supported by in vitro data showing that the α1a-AR subtype engages PKC signaling more potently than α1d-ARs (Taguchi et al., 1998). However, there are currently no α1d-AR-selective compounds that would allow testing of this hypothesis.
Species comparisons for rat versus monkey immuno-EM α1-ARs
The general pattern of α1-AR expression at the ultrastructural level is schematically illustrated in Figure 9B and is remarkably similar between rat mPFC (layer V/VI) and monkey dlPFC layer III. The Weinshenker group (Mitrano et al., 2012, 2014) has described α1-AR labeling in rat mPFC on unmylinated axons and axon terminals and on spines and dendrites, with most of the dendritic labeling in the cytoplasm likely trafficking to more distal aspects of the cell. A similar pattern was seen in monkey, with axonal labeling in both nonterminal and terminal regions and the spine labeling occasionally within the PSD, but mostly distant from the synapse often near the calcium-containing spine apparatus. α1-AR in the PSD may phosphorylate NMDARs to maintain receptors in the synapse and promote excitatory actions, as suggested by physiological recordings in rat mPFC (Luo et al., 2014), whereas α1-ARs more distant from the PSD may engage calcium–cAMP-K+ channel actions that reduce firing (Datta and Arnsten, 2018).
A key difference between the rat and monkey was the pronounced expression of α1-ARs in glia (likely astrocytes) in monkey layer III dlPFC, but not in rat mPFC. α1-ARs have been shown to mediate the calcium waves that coordinate excitation across wide cortical regions (Ding et al., 2013), which may play a larger role in the expanded primate cortex.
Species comparisons for rat versus monkey α1-AR physiology
Direct comparisons of α1-AR actions in rat versus monkey PFC are challenging due to a number of factors. The PFC subregion under investigation is different (mPFC in rats vs dlPFC in monkeys) and the physiological studies in rat mPFC are from in vitro slice recordings from juvenile animals (P20–P23), usually from layer V neurons, under conditions in which there is no endogenous NE release. Therefore, the ascending side of the inverted-U dose response curve would be accentuated in these preparations and, indeed, these studies find excitatory effects of α1-AR stimulation involving both increased presynaptic glutamate release (Zhang et al., 2013; Luo et al., 2014) and facilitation of postsynaptic NMDAR and AMPAR actions (Luo et al., 2014). In vitro studies of rat mPFC have also found that α1-AR can reduce glutamatergic drive on GABA interneurons (Wang et al., 2013a), which may disinhibit cortex.
In contrast, the monkey studies are in vivo recordings from delay cells (likely layer III) from the dlPFC of adult, cognitively engaged animals with active NE release. The current study found the first evidence of endogenous NE having excitatory actions at α1-AR in monkey dlPFC and the immuno-EM suggests these may involve some of the same subcellular actions as in rat, for example, enhancing glutamate release and promoting NMDAR actions in the PSD. However, additional α1-AR stimulation with PE and especially cirazoline suppressed delay cell firing, suggesting that our monkeys are typically performing with optimal levels of NE α1-AR stimulation and that excessive activation suppresses firing. These cellular data explain why both PE infusion directly into monkey dlPFC (Mao et al., 1999) or systemic administration of cirazoline (Arnsten and Jentsch, 1997) impairs working memory performance in monkeys. Previous research indicates that these impairing actions involve calcium–PKC signaling (Birnbaum et al., 2004), which can drive feedforward calcium–PKC–cAMP signaling and reduce firing through opening of K+ channels in layer III dlPFC spines (Datta and Arnsten, 2018) (Fig. 9B).
Overall, the excitatory versus suppressive postsynaptic effects of α1-AR–calcium–PKC signaling in spines may depend upon the level of K+ channel expression and the levels of cytosolic calcium in that spine. Therefore, α1-AR stimulation may be predominately excitatory in spines with relatively few calcium-cAMP-sensitive K+ channels (SK, HCN, KCNQ) and with healthy calcium regulation, but may be predominately suppressive in spines where there are high levels of these K+ channels (e.g., in layer III dlPFC; Arnsten, 2015) and/or dysregulated calcium signaling (e.g., due to inflammation or advanced age; Oh et al., 2013; Arnsten, 2015). Therefore, neurons excited by PE may have received less endogenous NE stimulation or may not have had the postsynaptic signaling machinery, for example, KCNQ channel expression, needed to suppress firing.
Clinical relevance
The evidence of an α1-AR inverted-U influence on dlPFC physiology is consistent with the contrasting roles of α1-AR mechanisms in treatments for mental disorders, in which increased α1-AR stimulation can contribute to medications treating under-aroused subjects, whereas α1-AR blockade is central to treating stress-related disorders such as PTSD.
Both animal and human studies suggest that medications to promote vigilance can involve α1-AR stimulation, although the effects are subtle. For example, methylphenidate's enhancement of sustained attention in rats is reduced by α1-AR blockade (Berridge et al., 2012). Similarly, an α1-AR antagonist blocks the improvement in executive function induced by modafinil in healthy human subjects (Winder-Rhodes et al., 2010). Modafinil is used to treat narcolepsy and fatigue (Murillo-Rodríguez et al., 2018). Therefore, boosting α1-AR actions may help subjects with insufficient, endogenous NE actions.
In contrast, α1-AR antagonists are now in widespread use for disorders associated with stress and excessive NE signaling. Stress worsens or causes a variety of disorders associated with PFC dysfunction, including depression, bipolar disorder, schizophrenia, and PTSD (Mazure, 1995). Animal studies also indicate that traumatic brain injury (TBI) may involve increased catecholamine release and α1-AR stimulation in PFC as a key etiological event (Kobori et al., 2006, 2011). There is some evidence of excessive NE α1-ARs in the dorsal PFC of suicide patients (Arango et al., 1993) and extensive evidence that PTSD involves excessive NE signaling (Southwick et al., 1993, 1999; O'Donnell et al., 2004).
Many treatments for stress-related disorders have α1-AR-blocking properties. For example, atypical antipsychotic medications are used to treat PTSD and as adjunct therapy in treatment-resistant depression (Stein et al., 2002; Adetunji et al., 2005; Zhou et al., 2015). In addition to blocking D2R and 5HT2R, atypical antipsychotics have potent α1-AR-blocking properties (e.g.: Ki values for clozapine: 19 nm, risperidone: 2 nm, olanzapine: 7 nm; Bymaster et al., 1996). Although the therapeutic role of α1-AR blockade is only rarely discussed (Baldessarini et al., 1992), the current data suggest that this property may play an important therapeutic role in rescuing dlPFC neurons from the suppressive effects of excessive α1-AR stimulation.
Finally, the α1-AR antagonist prazosin is widely used to treat PTSD in veterans, active duty soldiers, and civilians, including those with TBI (Raskind et al., 2003, 2013; Taylor et al., 2006; Ruff et al., 2012; Koola et al., 2014). Furthermore, the longer-acting α1-AR antagonist doxazosin is in experimental use (Smith and Koola, 2016). Although the initial focus of prazosin research was on reducing nightmares, data also show reduced daytime PTSD symptoms, especially in those taking higher doses or longer-acting treatments (Taylor et al., 2006; Ruff et al., 2012; Koola et al., 2014; Smith and Koola, 2016). Because PTSD symptomology is related to PFC dysfunction (Aupperle et al., 2012; Kühn and Gallinat, 2013; Fitzgerald et al., 2018), including weaker dlPFC regulation of emotional response (Aupperle et al., 2012), the current data suggest that restoration of dlPFC physiology with α1-AR blockade may be an important aspect of prazosin's therapeutic response. Although the current study used a spatial working memory task, dlPFC delay firing can also encode many aspects of top-down control, including affective value (Seo et al., 2007) and behavioral organization (Procyk and Goldman-Rakic, 2006). Therefore, α1-AR blockade may disinhibit dlPFC microcircuits during stress and allow generation of the representations needed to guide appropriate emotion and action in a healthy mental state.
Footnotes
This work was supported by the National Institutes of Health (Pioneer Award DP1AG047744-01 to A.F.T.A. and Grants DA02399 and EY002593 to P.R.). We thank Lisa Ciavarella, Tracy Sadlon, Sam Johnson, and Michelle Wilson for their technical expertise.
The authors declare no competing financial interests.
- Correspondence should be addressed to Min Wang at min.wang{at}yale.edu